Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2010 Feb;27(2-3):93-6.
doi: 10.1007/s10815-010-9385-8. Epub 2010 Feb 2.

Evaluation of the safety of time-lapse observations for human embryos

Tatsuo Nakahara  1 , Akira Iwase, Maki Goto, Toko Harata, Miyabi Suzuki, Miki Ienaga, Harumi Kobayashi, Sachiko Takikawa, Shuichi Manabe, Fumitaka Kikkawa, Hisao Ando
Affiliations

Evaluation of the safety of time-lapse observations for human embryos

Tatsuo Nakahara et al. J Assist Reprod Genet. 2010 Feb.

Abstract

Purpose: To assess the effects of light from an integrated optical microscope and evaluate the safety of time-lapse observations using a built-in microscope incubator.

Methods: We prospectively compared the fertilization rate and embryonic morphology after intracytoplasmic sperm injection between embryos cultured with time-lapse observations every 15 min in an incubator with an integrated optical microscope and embryos with intermittent observations (once a day) in conventional incubators.

Results: No significant differences were observed in the fertilization rate (57.5% vs. 57.5%) or the rate of excellent-good cleavage embryos (36.0% vs. 36.0%).

Conclusions: These results suggest that time-lapse observations using an incubator with an integrated optical microscope may therefore be safely utilized in clinical practice.

PubMed Disclaimer

References

    1. Ebner T, Yaman C, Moser M, Sommergruber M, Feichtinger O, Tews G. Prognostic value of first polar body morphology on fertilization rate and embryo quality in intracytoplasmic sperm injection. Hum Reprod. 2000;15(2):427–30. doi: 10.1093/humrep/15.2.427. - DOI - PubMed
    1. Jackson KV, Ginsburg ES, Hornstein MD, Rein MS, Clarke RN. Multinucleation in normally fertilized embryos is associated with an accelerated ovulation induction response and lower implantation and pregnancy rates in in vitro fertilization-embryo transfer cycles. Fertil Steril. 1998;70(1):60–6. doi: 10.1016/S0015-0282(98)00100-9. - DOI - PubMed
    1. Ludwig M, Schopper B, Al-Hasani S, Diedrich K. Clinical use of a pronuclear stage score following intracytoplasmic sperm injection: impact on pregnancy rates under the conditions of the German embryo protection law. Hum Reprod. 2000;15(2):325–9. doi: 10.1093/humrep/15.2.325. - DOI - PubMed
    1. Sakkas D, Shoukir Y, Chardonnens D, Bianchi PG, Campana A. Early cleavage of human embryos to the two-cell stage after intracytoplasmic sperm injection as an indicator of embryo viability. Hum Reprod. 1998;13(1):182–7. doi: 10.1093/humrep/13.1.182. - DOI - PubMed
    1. Scott LA, Smith S. The successful use of pronuclear embryo transfers the day following oocyte retrieval. Hum Reprod. 1998;13(4):1003–13. doi: 10.1093/humrep/13.4.1003. - DOI - PubMed

Publication types

MeSH terms

LinkOut - more resources