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. 2010 May;32(5):695-9.
doi: 10.1007/s10529-010-0206-z. Epub 2010 Feb 3.

Cholesterol biotransformation to androsta-1,4-diene-3,17-dione by growing cells of Chryseobacterium gleum

Priti N Chaudhari  1 , Bhushan L Chaudhari, Sudhir B Chincholkar
Affiliations

Cholesterol biotransformation to androsta-1,4-diene-3,17-dione by growing cells of Chryseobacterium gleum

Priti N Chaudhari et al. Biotechnol Lett. 2010 May.

Abstract

Cholesterol oxidase activity was studied during biotransformation of cholesterol to androsta-1,4-diene-3,17-dione (ADD) by Chryseobacterium gleum. Spent LB media, containing cholesterol (3 mM approximately = 1 g l(-1)) where the bacterium was grown for 24 h, at 30 degrees C with constant shaking at 120 rpm, had the highest enzyme activity (167 U mg(-1)). The growing cells produced 0.076 g ADD from 1 g cholesterol l(-1).

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