Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2010 Feb 3:7:26.
doi: 10.1186/1743-422X-7-26.

Inhibition of tomato yellow leaf curl virus (TYLCV) using whey proteins

Ashraf M Abdelbacki  1 , Soad H Taha, Mahmoud Z Sitohy, Abdelgawad I Abou Dawood, Mahmoud M Abd-El Hamid, Adel A Rezk
Affiliations

Inhibition of tomato yellow leaf curl virus (TYLCV) using whey proteins

Ashraf M Abdelbacki et al. Virol J. .

Retraction in

Abstract

The antiviral activity of native and esterified whey proteins fractions (alpha-lactalbumin, beta-lactoglobulin, and lactoferrin) was studied to inhibit tomato yellow leaf curl virus (TYLCV) on infected tomato plants. Whey proteins fractions and their esterified derivatives were sprayed into TYLCV-infected plants. Samples were collected from infected leaves before treatment, 7 and 15 days after treatment for DNA and molecular hybridization analysis. The most evident inhibition of virus replication was observed after 7 and 15 days using alpha-lactoferrin and alpha-lactalbumin, respectively. Native and esterified lactoferrin showed complete inhibition after 7 days. On the other hand, native beta-lactoglobulin showed inhibition after 7 and 15 days whereas esterified beta-lactoglobulin was comparatively more effective after 7 days. The relative amount of viral DNA was less affected by the esterified alpha-lactalbumin whereas native alpha-lactalbumin inhibited virus replication completely after 15 days. These results indicate that native or modified whey proteins fractions can be used for controlling the TYLCV-infected plants.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Antiviral activity of α-Lactalbumin (ALA). Antiviral activity of α-lactalbumin (ALA) on infected tomato plants treated with: A) native α-Lactalbumin, B) modified α-Lactalbumin (5 plants for each treatment). 1) Before treatment (zero time), 2) 7 days after treatment, 3) 15 days treatment. 4) Positive control (without treatment), 5) negative control (Healthy plants), 6) infected plants sprayed with water.
Figure 2
Figure 2
Antiviral activity of β-lactoglobulin (BLG). Antiviral activity of β-lactoglobulin ((BLG) on infected tomato plants treated with: A) native β-lactoglobulin, B) modified β-lactoglobulin (5 plants for each treatment). 1) Before treatment (zero time), 2) 7 days after treatment, 3) 15 days treatment. 4) Positive control (without treatment), 5) negative control (Healthy plants), 6) infected plants sprayed with water.
Figure 3
Figure 3
Antiviral activity of Lactoferrin. Antiviral activity of lactoferrin on infected tomato plants treated with: A) native lactoferrin, B) modified lactoferrin (5 plants for each treatment). 1) Before treatment (zero time), 2) 7 days after treatment, 3) 15 days treatment. 4) Positive control (without treatment), 5) negative control (Healthy plants), 6) infected plants sprayed with water.
See this image and copyright information in PMC

References

    1. Abdel-Salam AM. Isolation and characterization of a whitefly-transmitted geminivirus associated with the leaf curl and mosaic symptoms on cotton in. Egypt Arab J Biotech. 1999;2:193–218.
    1. Abdel-Salam AM, EI-Shazly AM, Thouvenel JC. Biological and biochemical studies on hollyhock leaf crumple virus (HLCrV): A newly discovered whitefly-transmitted geminivirus. Arab J Biotech. 1998;1:41–8.
    1. Abdel-Salam AM, Soliman ZD, EL-Banna MO. Characterization of a geminivirus infection tomato plant in Egypt. 3rd International Geminivirus symposium at John Innes Centre, Norwich, UK on 2001, 24-28, July.
    1. Fadl GM, Burgstaller H. Reduction of tomato leaf curl virus in Sudan through variety selection and insecticide application. Acta Horticulture. 1984;190:159–164.
    1. Fanigliulo A, Comes S, Crescenzi A, Momol MT, Olson SM, Sacchetti M, Ferrara L, Caligiuri G. Integrated management of tomato yellow leaf curl in protected tomato crops in southern Italy. Acta Horticulture (ISHS) 2009;808:393–396.

Publication types

MeSH terms

LinkOut - more resources