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Comparative Study
. 2010 Apr;48(4):1413-6.
doi: 10.1128/JCM.01409-09. Epub 2010 Feb 3.

Evaluation of an upgraded version of the Roche Cobas AmpliPrep/Cobas TaqMan HIV-1 test for HIV-1 load quantification

F Damond  1 , V Avettand-Fenoel, G Collin, B Roquebert, J C Plantier, A Ganon, D Sizmann, R Babiel, J Glaubitz, M L Chaix, F Brun-Vezinet, D Descamps, C Rouzioux
Affiliations
Comparative Study

Evaluation of an upgraded version of the Roche Cobas AmpliPrep/Cobas TaqMan HIV-1 test for HIV-1 load quantification

F Damond et al. J Clin Microbiol. 2010 Apr.

Abstract

We evaluated the performance of the prototype Cobas AmpliPrep/Cobas TaqMan HIV-1 test, version 2.0, using prospective and archived clinical samples initially underquantitated by the Cobas AmpliPrep/Cobas TaqMan HIV-1 test. The performance of the new test was significantly improved, and the majority of the underquantitation observed with the first-version test was eliminated.

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Figures

FIG. 1.
FIG. 1.
Bland-Altman plots for comparisons between CAP/CTM v1.0, CAP/CTM v2.0, and CA/HIM v1.5 in a panel of 25 archived plasma samples. (A) Bland-Altman plot for the comparison between CAP/CTM v1.0 and CA/HIM v1.5 in 25 archived samples. The broken line indicates the mean log10 difference, and the dotted lines represent the borders of the 95% confidence intervals (−2.29 and 0.62). (B) Bland-Altman plot for the comparison between CAP/CTM v2.0 and CA/HIM v1.5 in 25 archived samples. The broken line indicates the mean log10 difference, and the dotted lines represent the borders of the 95% confidence intervals (−0.79 and 1.12). (C) Bland-Altman plot for the comparison between CAP/CTM v1.0 and CAP/CTM v2.0 in 25 archived samples. The broken line indicates the mean log10 difference, and the dotted lines represent the borders of the 95% confidence intervals (−0.43 and 2.42).
FIG. 2.
FIG. 2.
Bland-Altman plots for comparisons between CAP/CTM v1.0, CAP/CTM v2.0, and CA/HIM v1.5 in a panel of prospectively collected plasma samples. (A) Bland-Altman plot for the comparison between CAP/CTM v1.0 and CA/HIM v1.5 in 259 valid result pairs for a panel of prospective routine clinical samples. The broken line indicates the mean log10 difference, and the dotted lines represent the borders of the 95% confidence intervals (−0.92 and 0.62). (B) Bland-Altman plot for the comparison between CAP/CTM v2.0 and CA/HIM v1.5 in 259 valid result pairs for a panel of prospective routine clinical samples. The broken line indicates the mean log10 difference, and the dotted lines represent the borders of the 95% confidence intervals (−0.50 and 0.65). (C) Bland-Altman plot for the comparison between CAP/CTM v1.0 and CAP/CTM v2.0 (HIV-1 v2.0) in 259 valid result pairs for a panel of prospective routine clinical samples. The broken line indicates the mean log10 difference, and the dotted lines represent the borders of the 95% confidence intervals (−0.46 and 0.90).
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