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. 2010 Jun;101(11):4096-103.
doi: 10.1016/j.biortech.2010.01.036. Epub 2010 Feb 4.

Expression and characterization of Bacillus licheniformis chitinase (ChiA), suitable for bioconversion of chitin waste

Chomphunuch Songsiriritthigul  1 , Sasithorn Lapboonrueng, Phornsiri Pechsrichuang, Puntarika Pesatcha, Montarop Yamabhai
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Expression and characterization of Bacillus licheniformis chitinase (ChiA), suitable for bioconversion of chitin waste

Chomphunuch Songsiriritthigul et al. Bioresour Technol. 2010 Jun.

Abstract

Chitinase (EC 3.2.1.14) is an enzyme with multiple industrial applications. These include bioconversion of chitin waste, a highly resistant and abundant biopolymer from crustacean food industry, into glucosamine and chito-oligosaccharide value-added products. This paper reports on the expression of endochitinase (ChiA) from Bacillus licheniformis strain DSM8785 in E. coli and characterization of the recombinant enzyme. Recombinant ChiA could efficiently convert colloidal chitin to N-acetyl glucosamine and chitobiose at pH 4.0, 6.0 and 9.0 at 50 degrees C and retained its activity up to 3days under these conditions, suggesting that this enzyme is suitable for bioconversion of chitin waste.

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