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Comment
. 2010 Feb 9;107(6):2379-80.
doi: 10.1073/pnas.0914955107. Epub 2010 Feb 1.

Finding enzymes that are actively involved in cancer

Matthew Bogyo  1
Affiliations
Comment

Finding enzymes that are actively involved in cancer

Matthew Bogyo. Proc Natl Acad Sci U S A. .
No abstract available

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Conflict of interest statement

The author declares no conflict of interest.

Figures

Fig. 1
Fig. 1
Activity-based proteomics or activity-based protein profiling (ABPP). In this example, tumor tissue samples are labeled with an activity-based probe (ABP) that contains a reactive fluorophosphonate group. After labeling of target enzymes (in this case serine hydrolases), labeled proteins are separated by SDS/PAGE, and relative activity levels are determined by the intensity of probe labeling. Potentially interesting targets are identified as having increased or reduced levels of activity in tumor samples. Labeled targets are isolated by affinity purification via the probe tag and identified by mass spectrometry.
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References

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