Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comment
. 2010 Feb 9;107(6):2379-80.
doi: 10.1073/pnas.0914955107. Epub 2010 Feb 1.

Finding enzymes that are actively involved in cancer

Matthew Bogyo  1
Affiliations
Comment

Finding enzymes that are actively involved in cancer

Matthew Bogyo. Proc Natl Acad Sci U S A. .
No abstract available

PubMed Disclaimer

Conflict of interest statement

The author declares no conflict of interest.

Figures

Fig. 1
Fig. 1
Activity-based proteomics or activity-based protein profiling (ABPP). In this example, tumor tissue samples are labeled with an activity-based probe (ABP) that contains a reactive fluorophosphonate group. After labeling of target enzymes (in this case serine hydrolases), labeled proteins are separated by SDS/PAGE, and relative activity levels are determined by the intensity of probe labeling. Potentially interesting targets are identified as having increased or reduced levels of activity in tumor samples. Labeled targets are isolated by affinity purification via the probe tag and identified by mass spectrometry.
See this image and copyright information in PMC

Comment on

References

    1. Shields DJ, et al. RBBP9—a tumor-associated serine hydrolase activity required for pancreatic neoplasia. Proc Nat. Acad Sci USA. 2010;107:2189–2194. - PMC - PubMed
    1. Evans MJ, Cravatt BF. Mechanism-based profiling of enzyme families. Chem Rev. 2006;106:3279–3301. - PubMed
    1. Fonovic M, Bogyo M. Activity-based probes as a tool for functional proteomic analysis of proteases. Expert. Re. Proteonomics. 2008;5:721–730. - PMC - PubMed
    1. Paulick MG, Bogyo M. Application of activity-based probes to the study of enzymes involved in cancer progression. Curr Opin Genet Dev. 2008;18:97–106. - PMC - PubMed
    1. Sadaghiani AM, Verhelst SH, Bogyo M. Tagging and detection strategies for activity-based proteomics. Curr Opin Chem Biol. 2007;11:20–28. - PubMed

MeSH terms

LinkOut - more resources