Phytoglycoprotein (75 kDa) suppresses release of histamine and expression of IL-4 and IFN- gamma in BPA-treated RBL-2H3 cells

Abstract

The current study investigated whether the phytoglycoprotein (75 kDa) isolated from Cudrania tricuspidata Bureau (CTB glycoprotein) has anti-allergic inflammatory potential. Bisphenol A (BPA) is well known to promote development of allergy-related immune dysfunction. This experiment evaluated the release of histamine, increased intracellular Ca(2+) level, and the activities of protein kinase C alpha (PKC alpha), transcription factors NFkappaB and cytokines (IL-4, and IFN-gamma) in BPA-treated RBL-2H3 cells. The results in this study showed that the phytoglycoprotein (75 kDa) inhibits the intracellular Ca(2+) level, translocation of PKC alpha from cytosol to membrane and the translocation of NF-kappaB in cells. We also found that the phytoglycoprotein (75 kDa) inhibits the release of histamine, and the expressions of cytokines (IL-4 and IFN-gamma) in the presence of BPA in RBL-2H3 cells. The results obtained from these experiments indicated that the phytoglycoprotein (75 kDa) inhibits release of histamine and expressions of IL-4, and IFN-gamma via down regulations of Ca(2+)/PKCalpha and NF-kappaB on the stage of degranulation induced by BPA. From point of view, we speculated that the phytoglycoprotein (75 kDa) is one of natural compounds that can block allergic-inflammation caused by BPA.