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. 2010 Jan 29;17(1):3-4.
doi: 10.1016/j.chembiol.2010.01.003.

On-bead library screening made easier

Dehua Pei  1
Affiliations

On-bead library screening made easier

Dehua Pei. Chem Biol. .

Abstract

In this issue of Chemistry & Biology, Astle et al. report the rapid identification of the most active hits from a large one-bead-one-compound peptoid library by magnetic sorting and without the need for labor-intensive resynthesis of the hits.

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Figures

Figure 1
Figure 1. OBOC library screening by magnetic bead sorting coupled with microarray analysis
Library beads incubated with the target protein are rendered as a suspension in a tube. A magnet is placed next to the tube and the beads are allowed to settle. Negative beads settle to the bottom while the hits remain on the wall of the tube.
See this image and copyright information in PMC

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