On-bead library screening made easier

Abstract

In this issue of Chemistry & Biology, Astle et al. report the rapid identification of the most active hits from a large one-bead-one-compound peptoid library by magnetic sorting and without the need for labor-intensive resynthesis of the hits.

Figure 1. OBOC library screening by magnetic bead sorting coupled with microarray analysis

Library beads incubated with the target protein are rendered as a suspension in a tube. A magnet is placed next to the tube and the beads are allowed to settle. Negative beads settle to the bottom while the hits remain on the wall of the tube.