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. 2010 Feb 5:16:161-6.

Differential expression of transforming growth factor-beta isoforms in bullous keratopathy corneas

Barbara Strzalka-Mrozik  1 , Agnieszka Stanik-Walentek, Malgorzata Kapral, Malgorzata Kowalczyk, Jolanta Adamska, Joanna Gola, Urszula Mazurek
Affiliations

Differential expression of transforming growth factor-beta isoforms in bullous keratopathy corneas

Barbara Strzalka-Mrozik et al. Mol Vis. .

Abstract

Purpose: The aim of this study was to investigate transcriptional activities of genes encoding transforming growth factor (TGF)-beta isoforms in bullous keratopathy corneas.

Methods: The study group consisted of 45 patients with bullous keratopathy (22 females and 23 males). The control group included 45 corneal donors (21 females and 24 males). Quantification of TGF-beta1, TGF-beta2, and TGF-beta3 mRNAs was performed by real-time quantitative reverse transcription PCR (QRT-PCR).

Results: TGF-beta1, TGF-beta2, and TGF-beta3 mRNAs were detected in both normal and pseudophakic bullous keratopathy (PBK) corneas. We found significantly lower transcriptional activity of TGF-beta3 mRNA in bullous keratopathy corneas compared to normal tissues. TGF-beta1 and TGF-beta2 expressions were at the same level in both PBK and healthy corneas.

Conclusions: Downregulation of TGF-beta3 gene expression may play a significant role in molecular changes observed in bullous keratopathy.

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Figures

Figure 1
Figure 1
Reverse transcription PCR products separated in 6% polyacrylamide gel. lane 1, marker of size pBR 322/BsuRI (MBI Fermentas, Vilnius, Lithuania); lane 2, transforming growth factor -β1 (152 base pair, bp); lane 3, transforming growth factor -β2 (201 bp); lane 4, transforming growth factor -β3 (121 bp); lane 5 glyceraldehyde-3-phosphate dehydrogenase (226 bp).
Figure 2
Figure 2
Transforming growth factor β in normal human corneas and pseudophakic bullous keratopathy corneas. The expression of transforming growth factor -β1, transforming growth factor -β2, and transforming growth factor -β3 isoforms in (A) normal human corneas (Kruskal–Wallis one-way analysis of variance test; p=0.0003) and (B) pseudophakic bullous keratopathy corneas (Kruskal–Wallis one-way analysis of variance test; p<0.0001). C: Comparison of transforming growth factor -β3 gene expression between pseudophakic bullous keratopathy and normal corneas (Mann–Whitney U test, p=0.0107).
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