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Review
. 2010 Feb;5(2):303-11.
doi: 10.2217/fmb.09.121.

The use of RNAi-based screens to identify host proteins involved in viral replication

Alec J Hirsch  1
Affiliations
Review

The use of RNAi-based screens to identify host proteins involved in viral replication

Alec J Hirsch. Future Microbiol. 2010 Feb.

Abstract

The recent development of RNAi-based techniques for protein knockdown in mammalian cells has allowed for unprecedented flexibility in the study of protein function. Currently, large siRNA libraries are available that allow the knockdown of all proteins known to be encoded by the human genome. These libraries have been used to identify the host proteins required for the replication of several clinically important viruses, including HIV, flaviviruses and influenza. This review summarizes the methods used in RNAi-based screening for host factors involved in virus replication, and discusses published examples of such screens.

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References

    1. Elbashir SM, Harborth J, Lendeckel W, Yalcin A, Weber K, Tuschl T. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature. 2001;411(6836):494–498. - PubMed
    1. Cherry S. What have RNAi screens taught us about viral–host interactions? Curr. Opin. Microbiol. 2009;12(4):446–452. - PMC - PubMed
    1. Goff SP. Knockdown screens to knockout HIV-1. Cell. 2008;135(3):417–420. - PMC - PubMed
    1. Reynolds A, Leake D, Boese Q, Scaringe S, Marshall WS, Khvorova A. Rational siRNA design for RNA interference. Nat. Biotechnol. 2004;22(3):326–330. - PubMed
    1. Westerhout EM, Berkhout B. A systematic analysis of the effect of target RNA structure on RNA interference. Nucleic Acids Res. 2007;35(13):4322–4330. - PMC - PubMed

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