Application of real-time PCR in Ghana, a Buruli ulcer-endemic country, confirms the presence of Mycobacterium ulcerans in the environment
- PMID: 20146745
- DOI: 10.1111/j.1574-6968.2010.01902.x
Application of real-time PCR in Ghana, a Buruli ulcer-endemic country, confirms the presence of Mycobacterium ulcerans in the environment
Abstract
This study reports the first successful application of real-time PCR for the detection of Mycobacterium ulcerans, the causative agent of Buruli ulcer (BU), in Ghana, a BU-endemic country. Environmental samples and organs of small mammals were analyzed. The real-time PCR assays confirmed the presence of M. ulcerans in a water sample collected in a BU-endemic village in the Ashanti Region.
Similar articles
-
A comparison of DNA extraction procedures for the detection of Mycobacterium ulcerans, the causative agent of Buruli ulcer, in clinical and environmental specimens.J Microbiol Methods. 2009 Feb;76(2):152-8. doi: 10.1016/j.mimet.2008.10.002. Epub 2008 Oct 17. J Microbiol Methods. 2009. PMID: 18973778
-
Factors enhancing the control of Buruli ulcer in the Bomfa communities, Ghana.Trans R Soc Trop Med Hyg. 2011 Aug;105(8):459-65. doi: 10.1016/j.trstmh.2011.05.003. Epub 2011 Jun 8. Trans R Soc Trop Med Hyg. 2011. PMID: 21652052
-
Distribution of Mycobacterium ulcerans in buruli ulcer endemic and non-endemic aquatic sites in Ghana.PLoS Negl Trop Dis. 2008 Mar 26;2(3):e205. doi: 10.1371/journal.pntd.0000205. PLoS Negl Trop Dis. 2008. PMID: 18365034 Free PMC article.
-
Buruli ulcer (Mycobacterium ulcerans infection).Trans R Soc Trop Med Hyg. 2008 Oct;102(10):969-78. doi: 10.1016/j.trstmh.2008.06.006. Epub 2008 Jul 26. Trans R Soc Trop Med Hyg. 2008. PMID: 18657836 Review.
-
Laboratory diagnosis of Buruli ulcer disease.Future Microbiol. 2010 Mar;5(3):363-70. doi: 10.2217/fmb.10.3. Future Microbiol. 2010. PMID: 20210548 Review.
Cited by
-
Linking the Mycobacterium ulcerans environment to Buruli ulcer disease: Progress and challenges.One Health. 2021 Aug 18;13:100311. doi: 10.1016/j.onehlt.2021.100311. eCollection 2021 Dec. One Health. 2021. PMID: 34485670 Free PMC article. Review.
-
Endemic infectious cutaneous ulcers syndrome in the Oti Region of Ghana: Study of cutaneous leishmaniasis, yaws and Haemophilus ducreyi cutaneous ulcers.PLoS One. 2023 Sep 27;18(9):e0292034. doi: 10.1371/journal.pone.0292034. eCollection 2023. PLoS One. 2023. PMID: 37756291 Free PMC article.
-
Understanding the transmission of Mycobacterium ulcerans: A step towards controlling Buruli ulcer.PLoS Negl Trop Dis. 2021 Aug 26;15(8):e0009678. doi: 10.1371/journal.pntd.0009678. eCollection 2021 Aug. PLoS Negl Trop Dis. 2021. PMID: 34437549 Free PMC article. Review.
-
Insertion sequence element single nucleotide polymorphism typing provides insights into the population structure and evolution of Mycobacterium ulcerans across Africa.Appl Environ Microbiol. 2014 Feb;80(3):1197-209. doi: 10.1128/AEM.02774-13. Epub 2013 Dec 2. Appl Environ Microbiol. 2014. PMID: 24296504 Free PMC article.
-
Detection of Mycobacterium ulcerans in the environment predicts prevalence of Buruli ulcer in Benin.PLoS Negl Trop Dis. 2012 Jan;6(1):e1506. doi: 10.1371/journal.pntd.0001506. Epub 2012 Jan 31. PLoS Negl Trop Dis. 2012. PMID: 22303498 Free PMC article.
Publication types
MeSH terms
LinkOut - more resources
Full Text Sources