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. 2010 Mar;304(2):191-4.
doi: 10.1111/j.1574-6968.2010.01902.x. Epub 2010 Jan 18.

Application of real-time PCR in Ghana, a Buruli ulcer-endemic country, confirms the presence of Mycobacterium ulcerans in the environment

Koen Vandelannoote  1 , Lies Durnez, Diana Amissah, Sophie Gryseels, Alfred Dodoo, Shirley Yeboah, Phyllis Addo, Miriam Eddyani, Herwig Leirs, Anthony Ablordey, Françoise Portaels
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Application of real-time PCR in Ghana, a Buruli ulcer-endemic country, confirms the presence of Mycobacterium ulcerans in the environment

Koen Vandelannoote et al. FEMS Microbiol Lett. 2010 Mar.

Abstract

This study reports the first successful application of real-time PCR for the detection of Mycobacterium ulcerans, the causative agent of Buruli ulcer (BU), in Ghana, a BU-endemic country. Environmental samples and organs of small mammals were analyzed. The real-time PCR assays confirmed the presence of M. ulcerans in a water sample collected in a BU-endemic village in the Ashanti Region.

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