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. 2009 Oct;2009(10):pdb.prot5307.
doi: 10.1101/pdb.prot5307.

Strain maintenance of Nasonia vitripennis (parasitoid wasp)

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Strain maintenance of Nasonia vitripennis (parasitoid wasp)

John H Werren et al. Cold Spring Harb Protoc. 2009 Oct.

Abstract

Nasonia is a complex of four closely related species of wasps with several features that make it an excellent system for a variety of genetic studies. These include a short generation time, ease of rearing, interfertile species, visible and molecular markers, and a sequenced genome. Furthermore, its parasitoid lifestyle allows investigations of questions relating to parasitoid/host dynamics, host preference, and specialist versus generalist biology. It also can serve as a behavior model for studies of courtship, male aggression and territoriality, female dispersal, and sex ratio control. This protocol describes standard rearing of Nasonia vitripennis strains on Sarcophaga bullata hosts.

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References

    1. Perrot-Minnot MJ, Guo LR, Werren JH. Single and Double Infections with Wolbachia in the Parasitic Wasp Nasonia vitripennis: Effects on Compatibility. Genetics. 1996;143:961–972. - PMC - PubMed
    1. Saunders DD. Larval Diapause of Maternal Origin: Induction of Diapause in Nasonia vitripennis (Walk.) (Hymenoptera: Pteromalidae) Journal of Experimental Biology. 1965;42:495.

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