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Comparative Study
. 2010 May;95(3):273-7.
doi: 10.1016/j.pbb.2010.01.015. Epub 2010 Feb 10.

Medial forebrain stimulation enhances intracranial nociception and attenuates morphine analgesia suggesting the existence of an endogenous opioid antagonist

Affiliations
Comparative Study

Medial forebrain stimulation enhances intracranial nociception and attenuates morphine analgesia suggesting the existence of an endogenous opioid antagonist

Conan Kornetsky et al. Pharmacol Biochem Behav. 2010 May.

Abstract

The purpose of this experiment was to test in the rat the hypotheses that activation of the brain reward system would attenuate the effects of intracranial nociceptive stimulation and would potentiate the antinociceptive effects of morphine. In this experiment pain (nociception) was generated by electrical stimulation of a brain pain pathway, the mesencephalic reticular formation (MRF) of the rat. Reward pathway stimulation was to the medial forebrain bundle at the level of the lateral hypothalamus (MFB-LH). Current thresholds for escape from MRF stimulation were determined using a modification of the psychophysical methods of limits. MRF stimulation was delivered concurrently with different intensities of non-contingent MFB-LH stimulation. The effects of morphine and saline were determined under all stimulation conditions. Contrary to expectation MFB-LH stimulation significantly lowered MRF stimulation escape thresholds. Morphine administration elevated MRF thresholds in the absence of MFB-LH stimulation. However, this effect was blocked by concurrent MFB-LH stimulation. These findings, which mimic the effects of the opiate antagonist naloxone, i.e., potentiating of pain and antagonism of morphine's analgesic effects, suggest the presence of an endogenous opiate receptor antagonist.

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Figures

Fig. 1
Fig. 1
Coronal sections showing electrode placements for MRF on left. MFB-LH on right (N=5). Sections shown are adapted from the atlas of Paxinos and Watson (2005).
Fig 2
Fig 2
Mean ± SE effects of low intensity level MFB stimulation on the nociceptive threshold. *p< 0.03 **p<0.01
Fig 3
Fig 3
Mean ± SE analgesic effects of morphine alone and saline treatment Between respective dose of morphine alone and saline treatment. *p< 0.07 **p<0.02 Between morphine alone and respective dose of morphine plus 5 μA of MFB stimulation. †p<0.06 ††p<0.01

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