Berberine attenuates pro-inflammatory cytokine-induced tight junction disruption in an in vitro model of intestinal epithelial cells
- PMID: 20149867
- DOI: 10.1016/j.ejps.2010.02.001
Berberine attenuates pro-inflammatory cytokine-induced tight junction disruption in an in vitro model of intestinal epithelial cells
Abstract
Maintenance of the mucosal barrier is a critical function of intestinal epithelial tight junction. Berberine is one of the main constituents of Coptidis rhizoma, which has been used for patients with gastrointestinal disorders for a long time. This study aimed to determine whether berberine could alleviate pro-inflammatory cytokine-induced intestinal epithelial tight junction damage. Caco-2 monolayers were treated with tumor necrosis factor-alpha and interferon-gamma to induce barrier dysfunction. The protective effect of berberine on tight junctions was analyzed by measuring the trans-epithelial electrical resistance (TEER). Transmission electron microscopy was used to observe the morphology of tight junction, and subcellular localization of tight junction proteins was investigated by immunofluorescence microscopy and Western blot. Berberine significantly prevented pro-inflammatory cytokines induced decrease in TEER. The distortion of tight junction morphology and redistribution of tight junction protein occludin was also prevented by berberine treatment. Western blot analysis demonstrated that berberine inhibit the dislocation of occludin from raft fractions to non-raft fractions in membrane microdomains of tight junctions. We concluded that berberine can ameliorate pro-inflammatory cytokines induced intestinal epithelia tight junction damage in vitro, and berberine may be one of the targeted therapeutic agents that can restore barrier function in intestinal disease states.
2010 Elsevier B.V. All rights reserved.
Similar articles
-
The effect of berberine in vitro on tight junctions in human Caco-2 intestinal epithelial cells.Fitoterapia. 2009 Jun;80(4):241-8. doi: 10.1016/j.fitote.2009.02.005. Epub 2009 Feb 23. Fitoterapia. 2009. PMID: 19243699
-
Blockade of hypoxia-inducible factor-1α by YC-1 attenuates interferon-γ and tumor necrosis factor-α-induced intestinal epithelial barrier dysfunction.Cytokine. 2011 Dec;56(3):581-8. doi: 10.1016/j.cyto.2011.08.023. Epub 2011 Sep 3. Cytokine. 2011. PMID: 21890376
-
Interferon-gamma and tumor necrosis factor-alpha disrupt epithelial barrier function by altering lipid composition in membrane microdomains of tight junction.Clin Immunol. 2008 Jan;126(1):67-80. doi: 10.1016/j.clim.2007.08.017. Epub 2007 Oct 26. Clin Immunol. 2008. PMID: 17964857
-
Tight junctions and cell-cell interactions.Methods Mol Biol. 2006;341:185-95. doi: 10.1385/1-59745-113-4:185. Methods Mol Biol. 2006. PMID: 16799199 Review.
-
Altered permeability in inflammatory bowel disease: pathophysiology and clinical implications.Curr Opin Gastroenterol. 2007 Jul;23(4):379-83. doi: 10.1097/MOG.0b013e32816aa392. Curr Opin Gastroenterol. 2007. PMID: 17545772 Review.
Cited by
-
Impact of deoxynivalenol and kaempferol on expression of tight junction proteins at different stages of Caco-2 cell proliferation and differentiation.RSC Adv. 2019 Oct 28;9(59):34607-34616. doi: 10.1039/c9ra06222j. eCollection 2019 Oct 23. RSC Adv. 2019. PMID: 35529998 Free PMC article.
-
Histidine Prevents Cu-Induced Oxidative Stress and the Associated Decreases in mRNA from Encoding Tight Junction Proteins in the Intestine of Grass Carp (Ctenopharyngodon idella).PLoS One. 2016 Jun 9;11(6):e0157001. doi: 10.1371/journal.pone.0157001. eCollection 2016. PLoS One. 2016. PMID: 27280406 Free PMC article.
-
Sieving characteristics of cytokine- and peroxide-induced epithelial barrier leak: Inhibition by berberine.World J Gastrointest Pathophysiol. 2016 May 15;7(2):223-34. doi: 10.4291/wjgp.v7.i2.223. World J Gastrointest Pathophysiol. 2016. PMID: 27190695 Free PMC article.
-
Berberine Ameliorates Brain Inflammation in Poloxamer 407-Induced Hyperlipidemic Rats.Int Neurourol J. 2019 Nov;23(Suppl 2):S102-110. doi: 10.5213/inj.1938216.108. Epub 2019 Nov 30. Int Neurourol J. 2019. PMID: 31795609 Free PMC article.
-
Probiotics, Prebiotics, and Phytogenic Substances for Optimizing Gut Health in Poultry.Microorganisms. 2022 Feb 8;10(2):395. doi: 10.3390/microorganisms10020395. Microorganisms. 2022. PMID: 35208851 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous