Elastic system fibers in rat incisor periodontal ligament--immunohistochemical study using sections of fresh-frozen un-demineralized tissues

Abstract

The aim of this study was to examine the localization and distribution of the components of elastic system fibers in the periodontal ligament of continuously erupting rat incisors in an effort to understand the mechanism of the eruption of the tooth. Sections of fresh-frozen, un-demineralized incisors of the rat mandible were prepared for immunohistochemical localization of elastin, fibrillin-2 and microfibril-associated glycoprotein-1 (MAGP-1). The structure of the periodontal ligament was well preserved in sections of fresh-frozen tissues. At the basal region of the ligament, intense immunolabelling for fibrillin-2 and MAGP-1 was observed as dot-like structures (transversely sectioned fibers) mainly on the tooth side of the ligament close to the cementum. These dot-like structures gradually increased in number towards the incisal area and were distributed throughout the tooth side of the ligament. This pattern of distribution was the same as that of reported oxytalan fibers. Elastin-immunopositive fibers were also detected in the ligament, although the labelling was limited and distribution was sparse. In conclusion, both fibrillin-2 and MAGP-1 immunopositive fibers may serve as a scaffold for deposition of tropoelastin during elastogenesis in the periodontal ligament. They may also provide guidance for the migration of fibroblasts to the occlusive side, which generates contractile forces for the movement of the tooth for continuous eruption of incisors.