Minichromosome stability induced by partial genome duplication in Arabidopsis thaliana

Abstract

Two partially reconstructed karyotypes (RK1 and RK2) of Arabidopsis thaliana have been established from a transformant, in which four structurally changed chromosomes (alpha, beta, gamma, and delta) were involved. Both karyotypes are composed of 12 chromosomes, 2n = 1" + 3" + 4" + 5" + alpha" + gamma" = 12 for RK1 and 2n = 3" + 4" + 5" + alpha" + beta" + gamma" = 12 for RK2, and these chromosome constitutions were relatively stable at least for three generations. Pairing at meiosis was limited to the homologues (1, 3, 4, 5, alpha, beta, or gamma), and no pairing occurred among non-homologous chromosomes in both karyotypes. For minichromosome alpha (mini alpha), precocious separation at metaphase I was frequently observed in RK2, as found for other minichromosomes, but was rare in RK1. This stable paring of mini alpha was possibly caused by duplication of the terminal tip of chromosome 1 that is characteristic of RK1.