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. 2010 Apr;76(7):2371-5.
doi: 10.1128/AEM.02250-09. Epub 2010 Feb 12.

Role of Geobacter sulfurreducens outer surface c-type cytochromes in reduction of soil humic acid and anthraquinone-2,6-disulfonate

James W Voordeckers  1 , Byoung-Chan Kim, Mounir Izallalen, Derek R Lovley
Affiliations

Role of Geobacter sulfurreducens outer surface c-type cytochromes in reduction of soil humic acid and anthraquinone-2,6-disulfonate

James W Voordeckers et al. Appl Environ Microbiol. 2010 Apr.

Abstract

Deleting individual genes for outer surface c-type cytochromes in Geobacter sulfurreducens partially inhibited the reduction of humic substances and anthraquinone-2,6,-disulfonate. Complete inhibition was obtained only when five of these genes were simultaneously deleted, suggesting that diverse outer surface cytochromes can contribute to the reduction of humic substances and other extracellular quinones.

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Figures

FIG. 1.
FIG. 1.
Impact of deletion of single c-type cytochrome genes on reduction of AQDS (left panels) and the Elliott soil humic acid standard (right panels) for strains with the (a) omcS, (b) omcE, (c) omcB, or (d) omcZ gene deleted. The rates of reduction for the mutant strains were compared with the rate of reduction for a batch of wild-type cells grown and analyzed simultaneously. Incubation was carried out with acetate as the electron donor and (in controls) without added acetate. The data are the means ± standard errors for triplicate incubations.
FIG. 2.
FIG. 2.
Impact of deletion of the gene for OmpB (a) or a gene that affects its secretion to the outer surface, oxpG (b). Results for AQDS (left panels) and the Elliott soil humic acid standard (right panels) are shown. The data are the means ± standard errors for triplicate incubations.
FIG. 3.
FIG. 3.
Impact of deletion of multiple c-type cytochromes on reduction of AQDS (left panels) and the Elliott soil humic acid standard (right panels). The strains evaluated were deficient in the genes for (a) OmcB and OmcS; (b) OmcB, OmcE, OmcS, and OmcT; or (c) OmcB, OmcE, OmcS, OmcT, and OmcZ. The data are the means ± standard errors for triplicate incubations.
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