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. 2010 Feb;98(1):26-30.
doi: 10.1007/s10266-009-0112-9. Epub 2010 Feb 16.

Three-dimensional observation of the mouse embryo by micro-computed tomography: composition of the trigeminal ganglion

Hidekazu Aoyagi  1 , Kohzo Tsuchikawa, Shin-ichi Iwasaki
Affiliations

Three-dimensional observation of the mouse embryo by micro-computed tomography: composition of the trigeminal ganglion

Hidekazu Aoyagi et al. Odontology. 2010 Feb.

Abstract

The purpose of this study was to demonstrate a micro-computed tomography (CT) method for observations of the mouse embryo. At 13.0 days post-coitum, mouse embryos were fixed in 4% paraformaldehyde for 24 h and stained en bloc by osmium tetroxide overnight. The embryos were then embedded in paraffin using standard methods for 24 h. Specimens were analyzed by micro-CT and image processing was performed. Organs containing nervous and blood systems could be viewed as a result of different osmium-staining densities. The trigeminal ganglion was imaged using three-dimensional techniques. Observation of the embryo was possible by micro-CT with osmium tetroxide staining.

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