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. 2010 Jan 29;15(1):44-6.
doi: 10.1186/2047-783x-15-1-44.

Absolute count of T and B lymphocyte subsets is decreased in systemic sclerosis

Thilo Gambichler  1 , C Tigges, B Burkert, S Höxtermann, P Altmeyer, A Kreuter
Affiliations

Absolute count of T and B lymphocyte subsets is decreased in systemic sclerosis

Thilo Gambichler et al. Eur J Med Res. .

Abstract

Background: Previous reports on lymphocyte subpopulations in systemic sclerosis (SSc) are conflicting. Therefore, we aimed to investigate the lymphocyte subsets in SSc patients who were not on immunosuppressive therapy.

Methods: Lymphocyte subsets were assessed in the peripheral blood of SSc patients (n = 29) and healthy controls (n = 29) using the four colour flow cytometry method. Correlation studies were also performed in order to assess the relationship between lymphocyte subsets and clinical parameters.

Results: The absolute count of lymphocytes (P = 0.0042), CD3+ (P = 0.0014), CD4+ (P = 0.0070), CD8+ (P = 0.021), and CD19+ cells (P = 0.024) was significantly decreased in SSc patients when compared to healthy controls. CD4+/CD8+ ratio and the absolute count of CD56+ cells observed in SSc patients did not significantly differ from controls (P=0.165; P = 0.632, respectively). There was no substantial relationship between the lymphocyte subset levels and clinical features (i.e., SSc subtype, autoantibody profiles, organ involvement), except for a significant inverse correlation of CD19+ cells and the modified Rodnan skin score (r = -0.43, P = 0.020).

Conclusion: Our data support previous reports indicating that subsets of T lymphocytes as well as B lymphocytes play a role in the pathogenesis of SSc.

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References

    1. Leroy EC, Black C, Fleischmajer R, Jablonska S, Krieg T. et al.Scleroderma (systemic sclerosis): classification, subsets and pathogenesis. J Rheumatol. 1988;15:202–205. - PubMed
    1. Melendro EI, Saldate C, Rivero SJ, Alarcon-Segovia D. T-cell subpopulations in the peripheral blood of patients with connective tissue diseases as determined by flow cytometry using monoclonal antibodies. Clin Immunol Immunopathol. 1983;27:340–347. doi: 10.1016/0090-1229(83)90086-7. - DOI - PubMed
    1. Dalkiliç E, Dilek K, Güllülü M, Yavuz M, Karakoç Y, Yurtkuran M. et al.Lymphocyte phenotypes in systemic sclerosis. Ann Rheum Dis. 1999;58:719–720. - PMC - PubMed
    1. Kahan A, Kahan A, Picard F, Menkès CJ, Amor B. Abnormalities of T lymphocyte subsets in systemic sclerosis demonstrated with anti-CD45RA and anti-CD29 monoclonal antibodies. Ann Rheum Dis. 1991;50:354–358. doi: 10.1136/ard.50.6.354. - DOI - PMC - PubMed
    1. Gorla R, Airò P, Malagoli A, Carella G, Prati E, Brugnoni D, Franceschini F, Cattaneo R. CD4+ and CD8+ subsets: naive and memory cells in the peripheral blood of patients with systemic sclerosis. Clin Rheumatol. 1994;13:83–87. doi: 10.1007/BF02229871. - DOI - PubMed