Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2008 Jan 22;5(2):213-22.
doi: 10.4314/ajtcam.v5i2.31276.

Clerodendron inerme protects cellular integrity during 7,12-dimethylbenz[A]-anthracene induced hamster buccal pouch carcinogenesis

Shanmugam Manoharan  1 , Kannan Kavitha, Subramanian Balakrishnan, Kasinathan Rajalingam
Affiliations

Clerodendron inerme protects cellular integrity during 7,12-dimethylbenz[A]-anthracene induced hamster buccal pouch carcinogenesis

Shanmugam Manoharan et al. Afr J Tradit Complement Altern Med. .

Abstract

Aim of the present study was to investigate the protective effect of Clerodendron inerme on cellular integrity by measuring the status of glycoconjugates, lipids, osmotic fragility, and membrane bound enzyme activity in 7,12-dimethylbenz (a) anthracene (DMBA)-induced oral carcinogenesis. Oral squamous cell carcinoma was induced in the buccal pouch of Syrian golden hamsters by painting with 0.5% DMBA in liquid paraffin thrice a week for 14 weeks. The levels of glycoconjugates, lipids, osmotic fragility and membrane bound enzyme activity were analyzed by using specific colorimetric methods. We observed 100% tumor formation in DMBA painted hamsters. Altered glycoconjugates and lipid pattern were observed in DMBA painted hamsters as compared to control hamsters. Erythrocytes from DMBA painted hamsters were more fragile than those from control hamsters. The activity of membrane bound enzyme (Na(+) K(+) ATPase) decreased in DMBA painted hamsters as compared to control hamsters. Oral administration of aqueous leaf extract of Clerodendron inerme (CiALet) at a dose of 500 mg/kg body weight significantly prevented the tumor formation and histopathological abnormalities as well as normalized the above said biochemical variables in DMBA painted hamsters. Our results thus demonstrate the protective effect of Clerodendron inerme on cellular integrity during DMBA induced oral carcinogenesis.

Keywords: Clerodendron inerme; DMBA; Oral cancer; glycoconjugates; hamster; lipids; osmotic fragility.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Osmotic fragility curve of control and experimental hamsters in each group
Figure 2
Figure 2
Histopathological features of control and experimental hamsters in each group
See this image and copyright information in PMC

References

    1. Allampallam K, Dutt D, Nair C, Shetty V, Mundle S, Lisak L, Andrews C, Ahmed B, Mazzone L, Zorat F, Borok R, Muzammil M, Gundroo A, Ansaarie I, Raza A. The clinical and biologic significance of abnormal lipid profiles in patients with myelodysplastic syndromes. J Hematother Stem Cell Res. 2000;9:247–255. - PubMed
    1. Aranganathan S, Senthil K, Nalini N. A case control study of glycoprotein status in ovarian carcinoma. Clin Biochem. 2005;38:535–539. - PubMed
    1. Dische L, Shettles LB. Specific color reactions of methyl pentoses and spectrophotometric micromethod for their determination. J Biol Chem. 1948;175:595–604. - PubMed
    1. Dodge JF, Michell G, Hanahan DJ. The preparation of hemoglobin free ghosts of human red blood cells. Arch Biochem Biophys. 1968;110:119–130. - PubMed
    1. Falholf K, Falholf W, Lund B. An easy colorimetric method for routine determination of free fatty acid in plasma. Clin Chim Acta. 1973;46:105–111. - PubMed

LinkOut - more resources