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. 2010 Feb 17;6(1):1.
doi: 10.1186/1746-1448-6-1.

Culture independent molecular analysis of bacterial communities in the mangrove sediment of Sundarban, India

Abhrajyoti Ghosh #  1   2 , Nirmalya Dey #  1   3 , Amit Bera  1 , Amit Tiwari  1   4 , K B Sathyaniranjan  1 , Kalyan Chakrabarti  5 , Dhrubajyoti Chattopadhyay  1
Affiliations

Culture independent molecular analysis of bacterial communities in the mangrove sediment of Sundarban, India

Abhrajyoti Ghosh et al. Saline Syst. .

Abstract

Background: Sundarban is the world's largest coastal sediment comprising of mangrove forest which covers about one million hectares in the south-eastern parts of India and southern parts of Bangladesh. The microbial diversity in this sediment is largely unknown till date. In the present study an attempt has been made to understand the microbial diversity in this sediment using a cultivation-independent molecular approach.

Results: Two 16 S rRNA gene libraries were constructed and partial sequencing of the selected clones was carried out to identify bacterial strains present in the sediment. Phylogenetic analysis of partially sequenced 16 S rRNA gene sequences revealed the diversity of bacterial strains in the Sundarban sediment. At least 8 different bacterial phyla were detected. The major divisions of detected bacterial phyla were Proteobacteria (alpha, beta, gamma, and delta), Flexibacteria (CFB group), Actinobacteria, Acidobacteria, Chloroflexi, Firmicutes, Planctomycetes and Gammatimonadates.

Conclusion: The gammaproteobacteria were found to be the most abundant bacterial group in Sundarban sediment. Many clones showed similarity with previously reported bacterial lineages recovered from various marine sediments. The present study indicates a probable hydrocarbon and oil contamination in this sediment. In the present study, a number of clones were identified that have shown similarity with bacterial clones or isolates responsible for the maintenance of the S-cycle in the saline environment.

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Figures

Figure 1
Figure 1
16S rRNA gene tree showing positions of proteobacterial sequences in D16S_pMOS library including the reference sequences retrieved from GenBank. 16S rRNA gene sequence of Bacillus subtilis 168 is used to assign an out-group species.
Figure 2
Figure 2
16S rRNA gene tree showing positions of non-proteobacterial sequences (Flexibacteria, Planctomycetes, Firmicutes, Gemmatimonadates, unidentified/uncultured) in D16S_pMOS library including the reference sequences retrieved from GenBank. 16S rRNA gene sequence of Bacillus subtilis 168 is used to assign an out-group species.
Figure 3
Figure 3
16S rRNA gene tree showing positions of proteobacterial sequences in DUni_pMOS library including the reference sequences retrieved from GenBank. 16S rRNA gene sequence of Bacillus subtilis 168 is used to assign an out-group species.
Figure 4
Figure 4
16S rRNA gene tree showing positions of non-proteobacterial sequences (Actinobacteria, Planctomycetes, Chloroflexi, Acidobacteria, Gemmatimonadates, unidentified/uncultured) in D16S_pMOS library including the reference sequences retrieved from GenBank. 16S rRNA gene sequence of Bacillus subtilis 168 is used to assign an out-group species.
See this image and copyright information in PMC

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