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. 2010 Apr;48(4):1481-3.
doi: 10.1128/JCM.01780-09. Epub 2010 Feb 17.

Matrix-assisted laser desorption ionization-time of flight mass spectrometry for direct bacterial identification from positive blood culture pellets

Guy Prod'hom  1 , Alain Bizzini, Christian Durussel, Jacques Bille, Gilbert Greub
Affiliations

Matrix-assisted laser desorption ionization-time of flight mass spectrometry for direct bacterial identification from positive blood culture pellets

Guy Prod'hom et al. J Clin Microbiol. 2010 Apr.

Abstract

An ammonium chloride erythrocyte-lysing procedure was used to prepare a bacterial pellet from positive blood cultures for direct matrix-assisted laser desorption-ionization time of flight (MALDI-TOF) mass spectrometry analysis. Identification was obtained for 78.7% of the pellets tested. Moreover, 99% of the MALDI-TOF identifications were congruent at the species level when considering valid scores. This fast and accurate method is promising.

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Figures

FIG. 1.
FIG. 1.
Ammonium chloride procedure. A positive blood culture with S. aureus (aerobic vial) is shown along with a control that was processed in parallel without ammonium chloride (isotonic NaCl control). (A) Hemorrhagic pellets obtained after initial centrifugation, i.e., before the ammonium chloride step. (B) Pellet obtained after erythrocyte lysis with ammonium chloride (left tube) or with the isotonic NaCl control (right tube). (C) Bacterial pellet available for MALDI-TOF MS analysis (left tube). Please note the presence of a large numbers of erythrocytes in the pellet obtained without ammonium chloride (right tube).
See this image and copyright information in PMC

References

    1. Conway, G. C., S. C. Smole, D. A. Sarracino, R. D. Arbeit, and P. E. Leopold. 2001. Phyloproteomics: species identification of Enterobacteriaceae using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. J. Mol. Microbiol. Biotechnol. 3:103-112. - PubMed
    1. Friedrichs, C., A. C. Rodloff, G. S. Chhatwal, W. Schellenberger, and K. Eschrich. 2007. Rapid identification of viridans streptococci by mass spectrometric discrimination. J. Clin. Microbiol. 45:2392-2397. - PMC - PubMed
    1. Hsieh, S. Y., C. L. Tseng, Y. S. Lee, A. J. Kuo, C. F. Sun, Y. H. Lin, and J. K. Chen. 2008. Highly efficient classification and identification of human pathogenic bacteria by MALDI-TOF MS. Mol. Cell. Proteomics 7:448-456. - PubMed
    1. Mellmann, A., J. Cloud, T. Maier, U. Keckevoet, I. Ramminger, P. Iwen, J. Dunn, G. Hall, D. Wilson, P. Lasala, M. Kostrzewa, and D. Harmsen. 2008. Evaluation of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry in comparison to 16S rRNA gene sequencing for species identification of nonfermenting bacteria. J. Clin. Microbiol. 46:1946-1954. - PMC - PubMed
    1. Munson, E. L., D. J. Diekema, S. E. Beekmann, K. C. Chapin, and G. V. Doern. 2003. Detection and treatment of bloodstream infection: laboratory reporting and antimicrobial management. J. Clin. Microbiol. 41:495-497. - PMC - PubMed

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