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Comparative Study
. 2010 Jul 1;293(1):117-23.
doi: 10.1016/j.canlet.2010.01.003. Epub 2010 Feb 18.

Circulating tumor cells in metastatic colorectal cancer: efficacy and feasibility of different enrichment methods

Robert Königsberg  1 , Margit Gneist, Daniela Jahn-Kuch, Georg Pfeiler, Gudrun Hager, Marcus Hudec, Christian Dittrich, Robert Zeillinger
Affiliations
Comparative Study

Circulating tumor cells in metastatic colorectal cancer: efficacy and feasibility of different enrichment methods

Robert Königsberg et al. Cancer Lett. .

Abstract

Comprehensive in vitro and in vivo studies comparing EpCAM-based methods with other cytometric CTC enrichment technologies in metastatic colorectal cancer (mCRC) patients are lacking. We compare four manual cytometric methods to detect CTCs in vitro and in mCRC patients. The EpCAM-based technology, MACS HEA MicroBeads((R)), showed a significant better tumor cell recovery rate compared to other cytometric methods (p-value<0.0001). CTCs of 38 mCRC patients were enriched with MACS HEA MicroBeads(R). Progression-free survival did significantly differ between mCRC patients without detectable and with >or= 1 CTCs (p=0.007). CTC enrichment with EpCAM coupled antibodies is superior to other cytometric methods and is a feasible method for CTC detection in mCRC patients.

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