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. 1991 May;65(5):2711-3.
doi: 10.1128/JVI.65.5.2711-2713.1991.

High-efficiency formation of influenza virus transfectants

M Enami  1 , P Palese
Affiliations

High-efficiency formation of influenza virus transfectants

M Enami et al. J Virol. 1991 May.

Abstract

cDNA-derived RNAs were introduced into the genomes of influenza viruses by using an improved ribonucleoprotein (RNP) transfection protocol. Up to 10(5) viral transfectants with a novel neuraminidase gene could be obtained by using a 35-mm dish (10(6) cells) for RNP transfection. In addition to genes coding for surface proteins (hemagglutinin and neuraminidase), we also exchanged a gene coding for nonsurface proteins. The cDNA-derived influenza A/PR/8/34 virus NS gene was introduced into a temperature-sensitive mutant with a defect in this gene. We suggest that the term influenza virus transfectant be used for those viruses which are made by RNP transfection with cDNA-derived RNA.

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