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. 2010 May;38(5):431-6.
doi: 10.1016/j.jdent.2010.02.002. Epub 2010 Feb 18.

Solubility study of phytochemical cross-linking agents on dentin stiffness

Carina Strano Castellan  1 , Patricia N R Pereira, Grace Viana, Shao-Nong Chen, Guido F Pauli, Ana Karina Bedran-Russo
Affiliations

Solubility study of phytochemical cross-linking agents on dentin stiffness

Carina Strano Castellan et al. J Dent. 2010 May.

Abstract

Objectives: The effects of interactions between cross-linking proanthocyanidins (PA) in polar solvents and type-I collagen of demineralized dentin were investigated.

Methods: Three PA-rich extracts, two from grape seed (GSEP and GSES) and one from cocoa (COE), were dissolved (water, ethanol:water and acetone:water) and analyzed for their ability to increase the modulus of elasticity of demineralized dentin. Sound dentin beams (0.5mmx1.7mmx7mm) were fully demineralized and divided into 12 groups according to the type of cross-linking agent and solvents used. Specimens were immersed in the respective solutions and tested at baseline, 10, 30, 60, 120 and 240min.

Results: The elastic modulus (EM) of dentin was significantly increased by the PA treatment regardless of time (p<0.05 for all times). The extracts showed different solubility in different solvents. GSEP showed the highest increase in EM when diluted in distilled water and acetone at all exposure times. Both GSEs showed superior results when diluted in distilled water and after 4h of treatment, while COE produced strongest enhancement when dissolved in ethanol:water.

Conclusions: The results indicates that herbal extraction process and other pharmacognostic parameters have an important influence on extract solubility as well as constitution and, consequently, on the PA-dentin matrix interaction.

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Figures

Figure 1
Figure 1
Figure 1a. - Graph of the Apparent elastic modulus (MPa) values of demineralized dentin treated with grape seed extract from Mega-Natural; DW-distilled water; ET-ethanol-water; AC- acetone-water. 1b – Graph of the Apparent elastic modulus (MPa) over time of demineralized dentin treated with grape seed extract from Sanrisil; DW-distilled water; ET-ethanol-water; AC- acetone-water. 1c – Graph of the Apparent elastic modulus (MPa) over time of demineralized dentin treated with cocoa extract; DW-distilled water; ET-ethanol-water; AC- acetone-water. 1d – Graph of the Apparent elastic modulus (MPa) over time of demineralized dentin non treated; DW-distilled water; ET-ethanol-water; AC- acetone-water. NOTE: different scales in each graph.
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