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. 2009 Oct;41(5):227-32.
doi: 10.4103/0253-7613.58513.

In vitro antioxidant and antihyperlipidemic activities of Bauhinia variegata Linn

G P Rajani  1 , Purnima Ashok
Affiliations

In vitro antioxidant and antihyperlipidemic activities of Bauhinia variegata Linn

G P Rajani et al. Indian J Pharmacol. 2009 Oct.

Abstract

Objectives: To evaluate the ethanolic and aqueous extracts of Bauhinia variegata Linn. for in vitro antioxidant and antihyperlipidemic activity.

Materials and methods: Ethanolic and aqueous extracts of the stem bark and root of B. variegata Linn. were prepared and assessed for in vitro antioxidant activity by various methods namely total reducing power, scavenging of various free radicals such as 1,2-diphenyl-2-picrylhydrazyl (DPPH), super oxide, nitric oxide, and hydrogen peroxide. The percentage scavenging of various free radicals were compared with standard antioxidants such as ascorbic acid and butylated hydroxyl anisole (BHA). The extracts were also evaluated for antihyperlipidemic activity in Triton WR-1339 (iso-octyl polyoxyethylene phenol)-induced hyperlipidemic albino rats by estimating serum triglyceride, very low density lipids (VLDL), cholesterol, low-density lipids (LDL), and high-density lipid (HDL) levels.

Result: Significant antioxidant activity was observed in all the methods, (P < 0.01) for reducing power and (P < 0.001) for scavenging DPPH, super oxide, nitric oxide, and hydrogen peroxide radicals. The extracts showed significant reduction (P < 0.01) in cholesterol at 6 and 24 h and (P < 0.05) at 48 h. There was significant reduction (P < 0.01) in triglyceride level at 6, 24, and 48 h. The VLDL level was also significantly (P < 0.05) reduced from 24 h and maximum reduction (P < 0.01) was seen at 48 h. There was significant increase (P < 0.01) in HDL at 6, 24, and 48 h.

Conclusion: From the results, it is evident that alcoholic and aqueous extracts of B. variegata Linn. can effectively decrease plasma cholesterol, triglyceride, LDL, and VLDL and increase plasma HDL levels. In addition, the alcoholic and aqueous extracts have shown significant antioxidant activity. By the virtue of its antioxidant activity, B. variegata Linn. may show antihyperlipidemic activity.

Keywords: Antihyperlipidemic; Bauhinia variegata linn; antioxidant; triton.

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Figures

Figure 1
Figure 1
Comparison of hydrogen peroxide scavenging activity of 10 μg/ml of AA, BHA, BVRW, BVRA, BVSW and BVSA; (n = 3) AA: Ascorbic acid, BHA: Butylated hydroxy anisole; BVRW, BVRA, BVSW, BVSA: Bauhinia variegata root water, ethanolic and Bauhinia variegata stem water and ethanolic extracts, respectively
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