Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2010 Apr;48(4):1019-25.
doi: 10.1128/JCM.00219-10. Epub 2010 Feb 24.

Extended-spectrum-beta-lactamase, AmpC, and Carbapenemase issues

Kenneth S Thomson  1
Affiliations
Review

Extended-spectrum-beta-lactamase, AmpC, and Carbapenemase issues

Kenneth S Thomson. J Clin Microbiol. 2010 Apr.
No abstract available

PubMed Disclaimer

Figures

FIG. 1.
FIG. 1.
E. coli that produces an ESBL and a high level of AmpC. Shown is a CLSI ESBL confirmatory test on Mueller-Hinton agar supplemented with 200 μg/ml cloxacillin. No inhibition zones were obtained on unsupplemented Mueller-Hinton agar. The disks, from left to right, are as follows: upper, ceftazidime-clavulanate and ceftazidime; lower, cefotaxime-clavulanate and cefotaxime.
FIG. 2.
FIG. 2.
AmpC disk test with the upper Tris/EDTA disk inoculated with the test isolate according to the method of Black et al. (4) and placed adjacent to a cefoxitin (FOX) disk on a lawn of cefoxitin-susceptible E. coli. The indented zone margin is evidence of hydrolysis of cefoxitin by the AmpC enzymes that diffused out of the permeabilized cells on the Tris/EDTA disk.
See this image and copyright information in PMC

References

    1. Anderson, K. F., D. R. Lonsway, J. K. Rasheed, J. Biddle, B. Jensen, L. K. McDougal, R. B. Carey, A. Thompson, S. Stocker, B. Limbago, and J. B. Patel. 2007. Evaluation of methods to identify the Klebsiella pneumoniae carbapenemase in Enterobacteriaceae. J. Clin. Microbiol. 45:2723-2725. - PMC - PubMed
    1. Arakawa, Y., N. Shibata, K. Shibayama, H. Kurokawa, T. Yagi, H. Fujiwara, and M. Goto. 2000. Convenient test for screening metallo-beta-lactamase-producing gram-negative bacteria by using thiol compounds. J. Clin. Microbiol. 38:40-43. - PMC - PubMed
    1. Bhavnani, S. M., P. G. Ambrose, W. A. Craig, M. N. Dudley, and R. N. Jones. 2006. Outcomes evaluation of patients with ESBL- and non-ESBL-producing Escherichia coli and Klebsiella species as defined by CLSI reference methods: report from the SENTRY Antimicrobial Surveillance Program. Diagn. Microbiol. Infect. Dis. 54:231-236. - PubMed
    1. Black, J. A., E. S. Moland, and K. S. Thomson. 2005. AmpC disk test for detection of plasmid-mediated AmpC beta-lactamases in Enterobacteriaceae lacking chromosomal AmpC beta-lactamases. J. Clin. Microbiol. 43:3110-3113. - PMC - PubMed
    1. Bradford, P. A. 2001. Extended-spectrum beta-lactamases in the 21st century: characterization, epidemiology, and detection of this important resistance threat. Clin. Microbiol. Rev. 14:933-951. - PMC - PubMed

MeSH terms

LinkOut - more resources