Performance of a new chromogenic medium, BBL CHROMagar MRSA II (BD), for detection of methicillin-resistant Staphylococcus aureus in screening samples
- PMID: 20181915
- PMCID: PMC2849578
- DOI: 10.1128/JCM.02103-09
Performance of a new chromogenic medium, BBL CHROMagar MRSA II (BD), for detection of methicillin-resistant Staphylococcus aureus in screening samples
Abstract
Two chromogenic media for the detection of MRSA were compared: BBL CHROMagar MRSA II (BD) and MRSA ID agar (bioMérieux). Following overnight nonselective enrichment, 1,919 screening samples were inoculated on both chromogenic agars. After 24 h, the sensitivities of both media were high and comparable. Both media showed an important decrease in specificity after 48 h of incubation (decreases of 8% for MRSA II and 10% for MRSA ID), but MRSA II was significantly more specific at both time points.
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References
-
- CLSI. 2008. Performance standards for antimicrobial susceptibility testing. Eighteenth informational supplement. CLSI document M100-S18. Clinical and Laboratory Standards Institute, Wayne, PA.
-
- Davies, S., P. M. Zadik, C. M. Mason, and S. J. Whittaker. 2000. Methicillin-resistant Staphylococcus aureus: evaluation of five selective media. Br. J. Biomed. Sci. 57:269-272. - PubMed
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