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. 2010 Apr;48(4):1435-7.
doi: 10.1128/JCM.02417-09. Epub 2010 Feb 24.

Novel duplex real-time PCR assay detects Bordetella holmesii in specimens from patients with Pertussis-like symptoms in Ontario, Canada

J L Guthrie  1 , A V Robertson, P Tang, F Jamieson, S J Drews
Affiliations

Novel duplex real-time PCR assay detects Bordetella holmesii in specimens from patients with Pertussis-like symptoms in Ontario, Canada

J L Guthrie et al. J Clin Microbiol. 2010 Apr.

Abstract

Bordetella holmesii is a human pathogen found mainly in immunocompromised patients. A specific real-time PCR assay was developed and successfully used to identify specimens from which B. holmesii was misidentified as Bordetella pertussis and to establish the prevalence of B. holmesii in Ontario patients with pertussis-like symptoms.

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Figures

FIG. 1.
FIG. 1.
Multiple sequence alignment of a 50-bp segment of the recA gene for several Bordetella species. Real-time PCR primers designed for this study are underlined, and the TaqMan MGB probe is highlighted.
See this image and copyright information in PMC

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