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. 1991 Apr 1;275 ( Pt 1)(Pt 1):207-10.
doi: 10.1042/bj2750207.

Identification of a catalytically essential nucleophilic residue in sheep liver cytoplasmic aldehyde dehydrogenase

T M Kitson  1 , J P Hill, G G Midwinter
Affiliations

Identification of a catalytically essential nucleophilic residue in sheep liver cytoplasmic aldehyde dehydrogenase

T M Kitson et al. Biochem J. .

Abstract

Sheep liver cytoplasmic aldehyde dehydrogenase was labelled by reaction with the substrate p-nitrophenyl di[14C]methylcarbamate. After tryptic digestion and peptide fractionation the labelled residue was identified as Cys-302. This is the first unequivocal identification of the essential enzymic nucleophile in the esterase activity of aldehyde dehydrogenase. By implication, Cys-302 is probably also the residue that is acylated by aldehyde substrates and the first residue that is modified by disulfiram.

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References

    1. Biochem J. 1978 Oct 1;175(1):83-90 - PubMed
    1. Biochemistry. 1990 Feb 27;29(8):2070-5 - PubMed
    1. Biochemistry. 1982 Dec 21;21(26):6834-8 - PubMed
    1. Biochemistry. 1983 Aug 2;22(16):3784-91 - PubMed
    1. Eur J Biochem. 1984 May 15;141(1):21-35 - PubMed