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. 2010 Apr 2;12(7):1608-11.
doi: 10.1021/ol100338v.

Synthesis and evaluation of a new fluorescent transglycosylase substrate: lipid II-based molecule possessing a dansyl-C20 polyprenyl moiety

Chen-Yu Liu  1 , Chih-Wei Guo, Yi-Fan Chang, Jen-Tsung Wang, Hao-Wei Shih, Yu-Fang Hsu, Chia-Wei Chen, Shao-Kang Chen, Yen-Chih Wang, Ting-Jen R Cheng, Che Ma, Chi-Huey Wong, Jim-Min Fang, Wei-Chieh Cheng
Affiliations

Synthesis and evaluation of a new fluorescent transglycosylase substrate: lipid II-based molecule possessing a dansyl-C20 polyprenyl moiety

Chen-Yu Liu et al. Org Lett. .

Abstract

The preparation of a novel fluorescent lipid II-based substrate for transglycosylases (TGases) is described. This substrate has characteristic structural features including a shorter lipid chain, a fluorophore tag at the end of the lipid chain rather than on the peptide chain, and no labeling with a radioactive atom. This fluorescent substrate is readily utilized in TGase activity assays to characterize TGases and also to evaluate the activities of TGase inhibitors.

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