Microarray-based gene expression profiling and DNA copy number variation analysis of temporal fossa arachnoid cysts
- PMID: 20187927
- PMCID: PMC2841093
- DOI: 10.1186/1743-8454-7-6
Microarray-based gene expression profiling and DNA copy number variation analysis of temporal fossa arachnoid cysts
Abstract
Background: Intracranial arachnoid cysts (AC) are membranous sacs filled with CSF-like fluid that are commonly found in the temporal fossa. The majority of ACs are congenital. Typical symptoms are headache, dizziness, and dyscognition. Little is known about genes that contribute to the formation of the cyst membranes.
Methods: In order to identify differences in gene expression between normal arachnoid membrane (AM) and cyst membrane, we have performed a high-resolution mRNA microarray analysis. In addition we have screened DNA from AC samples for chromosomal duplications or deletions using DNA microarray-based copy number variation analysis.
Results: The transcriptome consisting of 33096 gene probes showed a near-complete similarity in expression between AC and AM samples. Only nine genes differed in expression between the two tissues: ASGR1, DPEP2, SOX9, SHROOM3, A2BP1, ATP10D, TRIML1, NMU were down regulated, whereas BEND5 was up regulated in the AC samples. Three of the AC samples had unreported human DNA copy number variations, all DNA gains.
Conclusions: Extending results of previous anatomical studies, the present study has identified a small subset of differentially expressed genes and DNA alterations in arachnoid cysts compared to normal arachnoid membrane.
Figures



Similar articles
-
Increased NKCC1 expression in arachnoid cysts supports secretory basis for cyst formation.Exp Neurol. 2010 Aug;224(2):424-8. doi: 10.1016/j.expneurol.2010.05.002. Epub 2010 May 21. Exp Neurol. 2010. PMID: 20471979
-
Protein profiling reveals inter-individual protein homogeneity of arachnoid cyst fluid and high qualitative similarity to cerebrospinal fluid.Fluids Barriers CNS. 2011 May 20;8:19. doi: 10.1186/2045-8118-8-19. Fluids Barriers CNS. 2011. PMID: 21599959 Free PMC article.
-
Quantitative proteomics comparison of arachnoid cyst fluid and cerebrospinal fluid collected perioperatively from arachnoid cyst patients.Fluids Barriers CNS. 2013 Apr 29;10(1):17. doi: 10.1186/2045-8118-10-17. Fluids Barriers CNS. 2013. PMID: 23628075 Free PMC article.
-
Intracranial arachnoid cysts--do they impair mental functions?J Neurol. 2008 Aug;255(8):1113-20. doi: 10.1007/s00415-008-0011-y. Epub 2008 Jul 28. J Neurol. 2008. PMID: 18677648 Review.
-
Sport-related structural brain injury associated with arachnoid cysts: a systematic review and quantitative analysis.Neurosurg Focus. 2016 Apr;40(4):E9. doi: 10.3171/2016.1.FOCUS15608. Neurosurg Focus. 2016. PMID: 27032926
Cited by
-
Neuropsychological improvement after posterior fossa arachnoid cyst drainage.Childs Nerv Syst. 2017 Jan;33(1):135-141. doi: 10.1007/s00381-016-3285-x. Epub 2016 Nov 10. Childs Nerv Syst. 2017. PMID: 27832354
-
Intracranial arachnoid cysts: impairment of higher cognitive functions and postoperative improvement.J Neurodev Disord. 2013 Aug 28;5(1):21. doi: 10.1186/1866-1955-5-21. J Neurodev Disord. 2013. PMID: 23985219 Free PMC article.
-
Familial arachnoid cysts: a review of 35 families.Childs Nerv Syst. 2019 Apr;35(4):607-612. doi: 10.1007/s00381-019-04060-z. Epub 2019 Jan 23. Childs Nerv Syst. 2019. PMID: 30673833
-
Dpep2 Emerging as a Modulator of Macrophage Inflammation Confers Protection Against CVB3-Induced Viral Myocarditis.Front Cell Infect Microbiol. 2019 Mar 7;9:57. doi: 10.3389/fcimb.2019.00057. eCollection 2019. Front Cell Infect Microbiol. 2019. PMID: 30899700 Free PMC article.
-
Mirror-image arachnoid cysts in a pair of monozygotic twins: a case report and review of the literature.Int J Med Sci. 2011;8(5):402-5. doi: 10.7150/ijms.8.402. Epub 2011 Jun 22. Int J Med Sci. 2011. PMID: 21750644 Free PMC article. Review.
References
-
- Sakai N, Kumagai M, Ueda T, Iwamura M, Nishimura Y, Miwa Y, Shimizu K, Hirata T, Andoh T, Yamada H. [Clinical study on intracranial arachnoid cyst: with reference to the middle cranial fossa] No Shinkei Geka. 1989;17:117–123. - PubMed
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials
Miscellaneous