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Comparative Study
. 2010 Mar 1:10:11.
doi: 10.1186/1471-2261-10-11.

Altered expression of microRNAs in the myocardium of rats with acute myocardial infarction

Bing Shi  1 , Yanhong Guo, Juan Wang, Wei Gao
Affiliations
Comparative Study

Altered expression of microRNAs in the myocardium of rats with acute myocardial infarction

Bing Shi et al. BMC Cardiovasc Disord. .

Abstract

Background: MicroRNAs(miRNAs) are important cellular components and their dysfunction is associated with various diseases. Acute myocardial infarction (AMI) is one of the most serious cardiovascular diseases. Although several miRNAs are reported to be associated with AMI, more novel miRNAs are needed to further investigate and improve certainty

Methods: We applied a well-established acute myocardial infarction rat model and performed miRNAs microarray experiments upon the myocardium tissue of rats with AMI and under sham control. We identified the differentially expressed miRNAs and analyzed the function of miRNA targets, transcription factors, and host genes based on bioinformatics.

Results: As a result, the levels of expression of seventeen miRNAs significantly deregulated, of which four miRNAs were further validated by qRT-PCR. In addition, we observed that the transcription factors, targets, and host genes of these deregulated miRNAs are enriched in cardiovascular-related functions.

Conclusion: We found that the miRNAs expression level altered in rats with AMI and differentially expressed miRNAs may be novel biomarkers of AMI.

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Figures

Figure 1
Figure 1
A heatmap of deregulated miRNAs in the myocardium of rats with AMI for three time points. A log-squared value of the fold change was imaged. Fold change values that were not available were imaged as a black color.
Figure 2
Figure 2
Deregulations of C-miRNAs and O-miRNAs. miRNAs show higher expression levels in normal cardiovascular tissues (C-miRNAs) tend to have higher probability of down-regulation in AMI compared with other miRNAs (O-miRNAs). "PDM" denotes the percentage of down-regulated miRNAs.
Figure 3
Figure 3
A comparison of fold change based on qRT-PCR and microarray analysis for four miRNAs.
See this image and copyright information in PMC

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