Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1991 May;59(5):1627-32.
doi: 10.1128/iai.59.5.1627-1632.1991.

Synthesis of tetanus toxin fragment C in insect cells by use of a baculovirus expression system

I G Charles  1 , B C Rodgers, A J Makoff, S N Chatfield, D E Slater, N F Fairweather
Affiliations

Synthesis of tetanus toxin fragment C in insect cells by use of a baculovirus expression system

I G Charles et al. Infect Immun. 1991 May.

Abstract

The baculovirus expression vector p36C was used to express in cells of the insect Spodoptera frugiperda fragment C of tetanus toxin under the control of the strong polyhedrin promoter. Fragment C was expressed intracellularly at a high level and was soluble, allowing it to be purified by affinity chromatography with monoclonal antibody TT08. Purified fragment C from baculovirus was used to immunize mice and was shown to successfully prevent the symptoms of tetanus following a toxin challenge. The ganglioside-binding properties of baculovirus-derived fragment C were compared with those of intact tetanus toxin and native fragment C and were found to be dissimilar.

PubMed Disclaimer

References

    1. Nucleic Acids Res. 1989 Jan 11;17(1):454 - PubMed
    1. Annu Rev Microbiol. 1988;42:177-99 - PubMed
    1. Adv Virus Res. 1988;35:177-92 - PubMed
    1. Proc Natl Acad Sci U S A. 1989 May;86(10):3554-8 - PubMed
    1. J Pharmacol Exp Ther. 1989 Sep;250(3):1043-7 - PubMed

LinkOut - more resources