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. 2010 Mar;7(3):188-90.
doi: 10.1038/nmeth0310-188.

aMAZe-ing tools for mosaic analysis in zebrafish

Jessica S Blackburn, David M Langenau

aMAZe-ing tools for mosaic analysis in zebrafish

Jessica S Blackburn et al. Nat Methods. 2010 Mar.
No abstract available

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Figures

Figure 1
Figure 1
Mosaic analysis in zebrafish. (a) Schematic of the current iteration of MAZe, with loxP sites denoted by blue triangles. (b) Schematic of the KalTA4-UAS Kaloop system. T2A is a sequence that allows bicistronic expression of two transgenes. (c) Future iterations of MAZe may include replacing the EF1α promoter with a truly ubiquitous promoter and GAL4-VP16 with KalTA4. A second Cre transgene driven by a tissue-specific promoter could be used to spatially target the MAZe transgene, and a tissue-specific and estrogen-responsive Cre (Cre-ER) would allow for spatiotemporal control of MAZe.
See this image and copyright information in PMC

References

    1. Collins R, Linker C, Lewis J. Nat. Methods. 2010;7:219–223. - PubMed
    1. Carmany-Rampey A, Moens C. Methods. 2006;39:228–238. - PubMed
    1. Shestopalov I, Sinha S, Chen J. Nat. Chem. Biol. 2007;3:650–651. - PubMed
    1. Langenau D, et al. Genes Dev. 2007;21:1382–1395. - PMC - PubMed
    1. Le X, et al. Proc. Natl. Acad. Sci. USA. 2007;104:9410–9415. - PMC - PubMed