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. 2010 Mar 3:10:67.
doi: 10.1186/1471-2180-10-67.

Development of O-antigen gene cluster-specific PCRs for rapid typing six epidemic serogroups of Leptospira in China

Cheng-Song Cai  1 , Yong-Zhang Zhu, Yi Zhong, Xiao-Fang Xin, Xiu-Gao Jiang, Xiao-Li Lou, Ping He, Jin-Hong Qin, Guo-Ping Zhao, Sheng-Yue Wang, Xiao-Kui Guo
Affiliations

Development of O-antigen gene cluster-specific PCRs for rapid typing six epidemic serogroups of Leptospira in China

Cheng-Song Cai et al. BMC Microbiol. .

Abstract

Background: Leptospira is the causative agent of leptospirosis. The O-antigen is the distal part of the lipopolysaccharide, which is a key component of outer membrane of Gram-negative bacteria and confers serological specificity. The epidemiology and clinical characteristics of leptospirosis are relative to the serology based taxonomic unit. Identification of Leptospira strains by serotyping is laborious and has several drawbacks.

Results: In this study, the O-antigen gene clusters of four epidemic Leptospira serogroups (serogroup Canicola, Autumnalis, Grippotyphosa and Hebdomadis) in China were sequenced and all genes were predicted in silico. Adding published sequences of two serogroups, Icterohaemorrhagiae (strain Lai and Fiocruz L1-130) and Sejroe (strain JB197 and L550), we identified six O-antigen-specific genes for six epidemic serogroups in China. PCR assays using these genes were developed and tested on 75 reference strains and 40 clinical isolates.

Conclusion: The results show that the PCR-based assays can be reliable and alternative means for rapid typing of these six serogroups of Leptospira.

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Figures

Figure 1
Figure 1
Analysis of amplification products by electrophoresis. Amplification products obtained by PCR of DNA pools from 18 serogroups belonging to Leptospira and DNA of two non-Leptospira strains using primer pairs ict-F/R (a), can-F/R (b), aut-F/R (c), gri-F/R (d). heb-F/R (e), sej-F/R (f). 1: Icterohaemorrhagiae; 2: Javanica; 3: Canicola; 4: Ballum; 5: Pyrogenes; 6: Autumnalis; 7: Australis; 8: Pomona; 9: Grippotyphosa; 10: Hebdomadis; 11: Bataviae; 12: Tarassovi; 13: Manhao; 14: Sejroe; 15: Mini; 16: Celledoni; 17: Ranarum; 18: Sarmin; 19: S. enteritidis H9812; 20: S. aureus N315; M: DNA marker, bands with lengths of 10 kb, 8 kb, 5 kb, 2 kb 1000 bp, 700 bp, 500 bp, 400 bp, 300 bp, 200 bp and 100 bp, respectively.
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References

    1. Faine S, Adler B, Bolin C, Perolat P. Leptospira and Leptospirosis. 2. Melbourne, Australia: MediSci; 1999.
    1. Brenner DJ, Kaufmann AF, Sulzer KR, Steigerwalt AG, Rogers FC, Weyant RS. Further determination of DNA relatedness between serogroups and serovars in the family Leptospiraceae with a proposal for Leptospira alexanderi sp. nov. and four new Leptospira genomospecies. International journal of systematic bacteriology. 1999;49(Pt 2):839–858. - PubMed
    1. Ramadass P, Jarvis BD, Corner RJ, Penny D, Marshall RB. Genetic characterization of pathogenic Leptospira species by DNA hybridization. International journal of systematic bacteriology. 1992;42:215–219. - PubMed
    1. Cerqueira GM, Picardeau M. A century of Leptospira strain typing. Infect Genet Evol. 2009;9:760–768. doi: 10.1016/j.meegid.2009.06.009. - DOI - PubMed
    1. Slack AT, Galloway RL, Symonds ML, Dohnt MF, Smythe LD. Reclassification of Leptospira meyeri serovar Perameles to Leptospira interrogans serovar Perameles through serological and molecular analysis: evidence of a need for changes to current procedures in Leptospira taxonomy. International journal of systematic and evolutionary microbiology. 2009;59:1199–1203. doi: 10.1099/ijs.0.000992-0. - DOI - PubMed

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