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. 2010 Mar 16;102(6):957-65.
doi: 10.1038/sj.bjc.6605594. Epub 2010 Mar 2.

A comprehensive evaluation of the accuracy of cervical pre-cancer detection methods in a high-risk area in East Congo

S Hovland  1 , M Arbyn, A K Lie, W Ryd, B Borge, E J Berle, H Skomedal, T M Kadima, L Kyembwa, E M Billay, D Mukwege, R B Chirimwami, T M Mvula, P J Snijders, C J L M Meijer, F Karlsen
Affiliations

A comprehensive evaluation of the accuracy of cervical pre-cancer detection methods in a high-risk area in East Congo

S Hovland et al. Br J Cancer. .

Abstract

Background: Given the high burden of cervical cancer in low-income settings, there is a need for a convenient and affordable method for detecting and treating pre-cancerous lesions.

Methods: Samples for comparing the accuracy of cytology, virology and histology were collected. Identification of HPV E6/E7 mRNA was performed using PreTect HPV-Proofer. HPV DNA detection was performed by GP5+/6+ PCR, followed by reverse line blot (RLB) for typing.

Results: A total of 343 women, aged 25-60 years, attending gynaecological polyclinics in DR Congo were included for sample enrolment. The test positivity rate was conventional and liquid-based cytology (LBC) at cutoff ASCUS+ of 6.9 and 6.6%, respectively; PreTect HPV-Proofer of 7.3%; and consensus DNA PCR for 14 HR types of 18.5%. Sixteen cases of CIN2+ lesions were identified. Of these, conventional cytology identified 66.7% with a specificity of 96.2%, LBC identified 73.3% with a specificity of 96.9%, all at cutoff ASCUS+. HR-HPV DNA detected all CIN2+ cases with a specificity of 85.9%, whereas PreTect HPV-Proofer gave a sensitivity of 81.3% and a specificity of 96.6%.

Conclusion: Both HPV detection assays showed a higher sensitivity for CIN2+ than did cytological methods. Detecting E6/E7 mRNA from only a subset of HR HPVs, as is the case with PreTect HPV-Proofer, resulted in a similar specificity to cytology and a significantly higher specificity than consensus HR HPV DNA (P<0.0001).

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Figures

Figure 1
Figure 1
Examination and sampling procedure. The symbols (1) and (2) indicate the order of examinations. The symbols (a) and (b) indicate that the cervix brush was first used for PAP smear preparation before the brush was submerged into 20 ml PreservCyt solution. ECC, endocervical curettage.
Figure 2
Figure 2
Overview of the material.
Figure 3
Figure 3
ROC diagram showing the accuracy (%) of all methods.
See this image and copyright information in PMC

References

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