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. 2008 Nov 3;3(1):Doc10.

In vivo analysis of wound healing by optical methods

Alena Alborova  1 , Jürgen Lademann, Axel Kramer, Heike Richter, Alexa Patzelt, Wolfram Sterry, Stefan Koch
Affiliations

In vivo analysis of wound healing by optical methods

Alena Alborova et al. GMS Krankenhhyg Interdiszip. .

Abstract
in English, German

The analysis of wound healing is important for the therapy control and for the development of drugs stimulating the healing process. Wounds cause damage to the skin barrier. A damaged stratum corneum leads to an increased water loss through the skin barrier. The standard measuring procedure for characterization of wound healing is the measurement of transepidermal water loss (TEWL). The disadvantage of this method is that it can be easily disturbed by the perspiration of the volunteers and by topically applied substances, for instance wound healing creams. In the study presented, in vivo laser scanning microscopy and optical coherent tomography were compared concerning the application for their analysis of wound healing processes. The laser scanning microscopy allows the analysis of the healing process on a cellular level. The course of wound healing determined by laser scanning microscopy was correlated with numerical values, allowing the numerical characterization of the wound healing process.

Die Analyse der Wundheilung ist wichtig zur Therapiekontrolle und für die Entwicklung von Arzneimitteln zur Wundheilungsförderung. Wunden verursachen eine Schädigung der Hautbarriere. Ein geschädigtes Stratum corneum verursacht einen erhöhten Wasserverlust durch die Hautbarriere. Daher ist das Standardverfahren zur Charakterisierung von Wundheilungsprozessen die Messung des transepidermalen Wasserverlusts (TEWL). Der Nachteil dieser Methode ist die Störanfälligkeit durch die Perspiration der Probanden sowie durch topisch applizierte Substanzen, z.B. Wundheilungscremes.

In der vorliegenden Studie werden die In-vivo-Laser-Scan-Mikroskopie und die optische Koheränztomographie in ihrer Aussagekraft, Wundheilungsprozesse zu charakterisieren, verglichen. Die In-vivo-Laser-Scan-Mikroskopie ermöglicht die Darstellung des Heilungsprozesses auf zellulärer Ebene. Mit Bewertungskriterien, die die Stadien des Wundheilungsprozesses beschreiben, lässt sich der Heilungsprozess numerisch beschreiben.

Keywords: in vivo laser scanning microscopy; optical coherent tomography; suction blister technique; transepidermal water loss; wound healing analysis.

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Figures

Figure 1
Figure 1. Comparison between a histological section (left picture) and an image obtained by OCT (right picture); the stratum corneum (SC), the epidermis (EP) and the dermis (DE) can be clearly recognized.
Figure 2
Figure 2. Comparison between a histological section (a) and an image obtained by LSM; in the LSM image the skin surface (b) and the boundary of the SC to the SG (c) can be clearly recognized.
Figure 3
Figure 3. LSM image showing the formation of a new cell layer of corneocytes during the wound healing process
See this image and copyright information in PMC

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