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. 2010 Apr;76(8):2681-3.
doi: 10.1128/AEM.02841-09. Epub 2010 Mar 5.

Heterologous expression of the oxytetracycline biosynthetic pathway in Myxococcus xanthus

D Cole Stevens  1 , Michael R Henry, Kimberly A Murphy, Christopher N Boddy
Affiliations

Heterologous expression of the oxytetracycline biosynthetic pathway in Myxococcus xanthus

D Cole Stevens et al. Appl Environ Microbiol. 2010 Apr.

Abstract

New natural products for drug discovery may be accessed by heterologous expression of bacterial biosynthetic pathways in metagenomic DNA libraries. However, a "universal" host is needed for this experiment. Herein, we show that Myxococcus xanthus is a potential "universal" host for heterologous expression of polyketide biosynthetic gene clusters.

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Figures

FIG. 1.
FIG. 1.
Oxytetracycline biosynthetic pathway. (A) Enzymatic pathway responsible for formation of oxytetracycline. (B) Oxytetracycline biosynthesis gene cluster from S. rimosus.
FIG. 2.
FIG. 2.
LC-MS ion extraction analysis of the molecular ion [M+H]+ of standard and culture extracts. (A) Oxytetracycline standard. (B) M. xanthus ΔasgE Kanr mutant containing the oxytetracycline biosynthetic pathway. (C) Wild-type M. xanthus DK1622.
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