AMP-activated protein kinase antagonizes pro-apoptotic extracellular signal-regulated kinase activation by inducing dual-specificity protein phosphatases in response to glucose deprivation in HCT116 carcinoma
- PMID: 20220132
- PMCID: PMC2863197
- DOI: 10.1074/jbc.M109.085456
AMP-activated protein kinase antagonizes pro-apoptotic extracellular signal-regulated kinase activation by inducing dual-specificity protein phosphatases in response to glucose deprivation in HCT116 carcinoma
Erratum in
-
Correction: AMP-activated protein kinase antagonizes pro-apoptotic extracellular signal-regulated kinase activation by inducing dual-specificity protein phosphatases in response to glucose deprivation in HCT116 carcinoma.J Biol Chem. 2018 Nov 16;293(46):18012. doi: 10.1074/jbc.AAC118.006426. J Biol Chem. 2018. PMID: 30446602 Free PMC article. No abstract available.
Abstract
Mitogen-activated protein kinase (MAPK) pathways are involved in the regulation of cellular responses, including cell proliferation, differentiation, cell growth, and apoptosis. Because these responses are tightly related to cellular energy level, AMP-activated protein kinase (AMPK), which plays an essential role in energy homeostasis, has emerged as another key regulator. In the present study, we demonstrate a novel signal network between AMPK and MAPK in HCT116 human colon carcinoma. Glucose deprivation activated AMPK and three MAPK subfamilies, extracellular signal-regulated kinase (ERK), c-Jun NH(2)-terminal kinase (JNK), and p38 MAPK. Under these conditions, inhibition of endogenous AMPK by expressing a dominant-negative form significantly potentiated ERK activation, indicating that glucose deprivation-induced AMPK is specifically antagonizing ERK activity in HCT116 cells. Moreover, we provide novel evidence that AMPK activity is critical for p53-dependent expression of dual-specificity phosphatase (DUSP) 1 & 2, which are negative regulators of ERK. Notably, ERK exhibits pro-apoptotic effects in HCT116 cells under glucose deprivation. Collectively, our data suggest that AMPK protects HCT116 cancer cells from glucose deprivation, in part, via inducing DUSPs, which suppresses pro-apoptotic ERK, further implying that a signal network between AMPK and ERK is a critical regulatory point in coupling the energy status of the cell to the regulation of cell survival.
Figures










Similar articles
-
Negative regulation of the LKB1/AMPK pathway by ERK in human acute myeloid leukemia cells.Exp Hematol. 2015 Jul;43(7):524-33.e1. doi: 10.1016/j.exphem.2015.03.005. Epub 2015 Apr 4. Exp Hematol. 2015. PMID: 25846811
-
Phospholipase D1 mediates AMP-activated protein kinase signaling for glucose uptake.PLoS One. 2010 Mar 9;5(3):e9600. doi: 10.1371/journal.pone.0009600. PLoS One. 2010. PMID: 20231899 Free PMC article.
-
Dysregulation of Dual-Specificity Phosphatases by Epstein-Barr Virus LMP1 and Its Impact on Lymphoblastoid Cell Line Survival.J Virol. 2020 Jan 31;94(4):e01837-19. doi: 10.1128/JVI.01837-19. Print 2020 Jan 31. J Virol. 2020. PMID: 31776277 Free PMC article.
-
Human Protein Kinases and Obesity.Adv Exp Med Biol. 2017;960:111-134. doi: 10.1007/978-3-319-48382-5_5. Adv Exp Med Biol. 2017. PMID: 28585197 Review.
-
The regulation of oncogenic Ras/ERK signalling by dual-specificity mitogen activated protein kinase phosphatases (MKPs).Semin Cell Dev Biol. 2016 Feb;50:125-32. doi: 10.1016/j.semcdb.2016.01.009. Epub 2016 Jan 11. Semin Cell Dev Biol. 2016. PMID: 26791049 Free PMC article. Review.
Cited by
-
CYP1A1 regulates breast cancer proliferation and survival.Mol Cancer Res. 2013 Jul;11(7):780-92. doi: 10.1158/1541-7786.MCR-12-0675. Epub 2013 Apr 10. Mol Cancer Res. 2013. PMID: 23576571 Free PMC article.
-
Impacts of autophagy-inducing ingredient of areca nut on tumor cells.PLoS One. 2015 May 27;10(5):e0128011. doi: 10.1371/journal.pone.0128011. eCollection 2015. PLoS One. 2015. PMID: 26017803 Free PMC article.
-
Metformin mediated reversal of epithelial to mesenchymal transition is triggered by epigenetic changes in E-cadherin promoter.J Mol Med (Berl). 2016 Dec;94(12):1397-1409. doi: 10.1007/s00109-016-1455-7. Epub 2016 Aug 17. J Mol Med (Berl). 2016. PMID: 27534967
-
High-Carbohydrate Diet Alleviates the Oxidative Stress, Inflammation and Apoptosis of Megalobrama amblycephala Following Dietary Exposure to Silver Nanoparticles.Antioxidants (Basel). 2021 Aug 25;10(9):1343. doi: 10.3390/antiox10091343. Antioxidants (Basel). 2021. PMID: 34572975 Free PMC article.
-
HSP90 inhibition in the mouse spinal cord enhances opioid signaling by suppressing an AMPK-mediated negative feedback loop.Sci Signal. 2023 Apr 11;16(780):eade2438. doi: 10.1126/scisignal.ade2438. Epub 2023 Apr 11. Sci Signal. 2023. PMID: 37040443 Free PMC article.
References
-
- Hardie D. G., Carling D., Carlson M. (1998) Annu. Rev. Biochem. 67, 821–855 - PubMed
-
- Inoki K., Ouyang H., Zhu T., Lindvall C., Wang Y., Zhang X., Yang Q., Bennett C., Harada Y., Stankunas K., Wang C. Y., He X., MacDougald O. A., You M., Williams B. O., Guan K. L. (2006) Cell 126, 955–968 - PubMed
-
- Jones R. G., Plas D. R., Kubek S., Buzzai M., Mu J., Xu Y., Birnbaum M. J., Thompson C. B. (2005) Mol. Cell 18, 283–293 - PubMed
-
- Xia Z., Dickens M., Raingeaud J., Davis R. J., Greenberg M. E. (1995) Science 270, 1326–1331 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
Miscellaneous