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. 2010 May;48(5):1884-7.
doi: 10.1128/JCM.02540-09. Epub 2010 Mar 10.

Rapid detection of influenza A pandemic (H1N1) 2009 virus neuraminidase resistance mutation H275Y by real-time reverse transcriptase PCR

Musa Hindiyeh  1 , Daniela Ram, Michal Mandelboim, Tal Meningher, Shira Hirsh, Jana Robinov, Virginia Levy, Sara Orzitzer, Roberto Azar, Zehava Grossman, Ella Mendelson
Affiliations

Rapid detection of influenza A pandemic (H1N1) 2009 virus neuraminidase resistance mutation H275Y by real-time reverse transcriptase PCR

Musa Hindiyeh et al. J Clin Microbiol. 2010 May.

Abstract

The emergence of oseltamivir-resistant influenza A pandemic (H1N1) 2009 virus highlights the need for rapid oseltamivir resistance screening. We report the development and validation of high-throughput real-time reverse transcriptase PCR assays for the detection of the H275Y substitution in the neuraminidase 1 gene that can be accomplished in 3 to 4 h.

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Figures

FIG. 1.
FIG. 1.
Linear limits of detection of the H275Y (A) and H275 (B) qRT-PCR assays. Serial (10-fold) dilutions of influenza A pandemic (H1N1) 2009 virus resistant (A) or sensitive (B) to oseltamivir were prepared and tested in qRT-PCR assays. Ct values were obtained for each dilution and are plotted against the TCID50 value.
See this image and copyright information in PMC

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