The maternally expressed Drosophila gene encoding the chromatin-binding protein BJ1 is a homolog of the vertebrate gene Regulator of Chromatin Condensation, RCC1
- PMID: 2022188
- PMCID: PMC452777
- DOI: 10.1002/j.1460-2075.1991.tb08064.x
The maternally expressed Drosophila gene encoding the chromatin-binding protein BJ1 is a homolog of the vertebrate gene Regulator of Chromatin Condensation, RCC1
Abstract
Using monoclonal antibodies I have identified a nuclear protein of Drosophila, BJ1 (Mr approximately 68 kd), and isolated its gene. Biochemical analysis demonstrates that the BJ1 protein is associated with nucleosomes and is released from chromatin by agents which intercalate into DNA, as previously shown for the high mobility group proteins (HMGs). On polytene chromosomes the protein is localized in all bands, with no preference for particular loci. Both the BJ1 protein and in particular the BJ1 mRNA are strongly expressed maternally. In early embryos all nuclei contain equal amounts of BJ1. During neuroblast formation, BJ1 mRNA becomes restricted to cells of the central nervous system, and higher protein levels are found in the nuclei of this tissue. In late embryonic stages, the mRNA almost completely disappears, but significant amounts of BJ1 protein persist until morphogenesis. The BJ1 gene encodes a 547 amino acid polypeptide featuring two different types of internal repeats. The sequence from amino acids 46 to 417 containing seven repeats of the first type has been highly conserved in evolution. 45% of the amino acids in this region are conserved in seven similar tandem repeats of the human gene Regulator of Chromatin Condensation, RCC1. The phenotype of a cell line carrying a mutation of RCC1 suggested a main function for this gene in cell cycle control. A yeast gene, SRM1/PRP20, also contains these repeats and shows 30% amino acid identity to BJ1 in this region. Mutations in this gene perturb mRNA metabolism, disrupt nuclear structure and alter the signal transduction pathway for the mating pheromone. Complementation experiments argue for a common function of these genes in the different species. I propose that their gene products bind to the chromatin to establish or maintain a proper higher order structure as a prerequisite for a regulated gene expression. Disruption of this structure could cause both mis-expression and default repression of genes, which might explain the pleiotropic phenotypes of the mutants.
Similar articles
-
Mutation of the hamster cell cycle gene RCC1 is complemented by the homologous genes of Drosophila and S.cerevisiae.EMBO J. 1991 May;10(5):1265-73. doi: 10.1002/j.1460-2075.1991.tb08068.x. EMBO J. 1991. PMID: 2022190 Free PMC article.
-
The Drosophila RCC1 homolog, Bj1, regulates nucleocytoplasmic transport and neural differentiation during Drosophila development.Dev Biol. 2004 Jun 1;270(1):106-21. doi: 10.1016/j.ydbio.2004.02.011. Dev Biol. 2004. PMID: 15136144
-
Analysis of yeast prp20 mutations and functional complementation by the human homologue RCC1, a protein involved in the control of chromosome condensation.Mol Gen Genet. 1991 Jul;227(3):417-23. doi: 10.1007/BF00273932. Mol Gen Genet. 1991. PMID: 1865879
-
RCC1 in the cell cycle: the regulator of chromosome condensation takes on new roles.Trends Biochem Sci. 1993 Mar;18(3):96-101. doi: 10.1016/0968-0004(93)90161-f. Trends Biochem Sci. 1993. PMID: 8480369 Review.
-
RCC1 in the Ran pathway.J Biochem. 1996 Aug;120(2):207-14. doi: 10.1093/oxfordjournals.jbchem.a021400. J Biochem. 1996. PMID: 8889801 Review.
Cited by
-
ChEC-seq2: an improved chromatin endogenous cleavage sequencing method and bioinformatic analysis pipeline for mapping in vivo protein-DNA interactions.NAR Genom Bioinform. 2024 Feb 7;6(1):lqae012. doi: 10.1093/nargab/lqae012. eCollection 2024 Mar. NAR Genom Bioinform. 2024. PMID: 38327869 Free PMC article.
-
Mutation of the hamster cell cycle gene RCC1 is complemented by the homologous genes of Drosophila and S.cerevisiae.EMBO J. 1991 May;10(5):1265-73. doi: 10.1002/j.1460-2075.1991.tb08068.x. EMBO J. 1991. PMID: 2022190 Free PMC article.
-
Chromosome condensation caused by loss of RCC1 function requires the cdc25C protein that is located in the cytoplasm.Mol Biol Cell. 1992 Dec;3(12):1373-88. doi: 10.1091/mbc.3.12.1373. Mol Biol Cell. 1992. PMID: 1337289 Free PMC article.
-
Pendulin, a Drosophila protein with cell cycle-dependent nuclear localization, is required for normal cell proliferation.J Cell Biol. 1995 Jun;129(6):1491-507. doi: 10.1083/jcb.129.6.1491. J Cell Biol. 1995. PMID: 7790350 Free PMC article.
-
Roles of Tubulin Concentration during Prometaphase and Ran-GTP during Anaphase of Caenorhabditis elegans Meiosis.Life Sci Alliance. 2024 Jul 3;7(9):e202402884. doi: 10.26508/lsa.202402884. Print 2024 Sep. Life Sci Alliance. 2024. PMID: 38960623 Free PMC article.
References
MeSH terms
Substances
Associated data
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
LinkOut - more resources
Full Text Sources
Molecular Biology Databases