Better preservation of Campylobacter jejuni/C. coli in a defined medium

Abstract

Campylobacter jejuni and C. coli strains were preserved at -10 degrees C in different stock media to determine their efficacy to preserve the organism for a longer period of time. An improved defined stock culture medium was developed for the organism by removal and effective neutralisation of the toxic metabolites. Comparative study revealed that phosphate buffer saline (PBS), pH 6.7 supplemented with 0.2 per cent charcoal, 0.025 per cent FBP (ferrous sulphate, sodium metabisulphate and sodium pyruvate), 0.1 per cent L-cystein and 10 per cent glycerol could support survival of C. jejuni coli strains for as long as 135 days at -10 degrees C followed by George's medium, brucella broth with 15 per cent glycerol, fetal calf serum with 50 per cent TSYB (tryplicase soy yeast-broth) and glycerol transport broth respectively.