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. 1977 Nov 1;167(2):501-4.
doi: 10.1042/bj1670501.

A simple radioassay for angiotensin-converting enzyme

J W Ryan, A Chung, C Ammons, M L Carlton

A simple radioassay for angiotensin-converting enzyme

J W Ryan et al. Biochem J. .

Abstract

Angiotensin-converting enzyme can be measured by the rate of release of 3H-labelled hippurate from p-[3H]benzoylglycylglycylglycine. The product is separable from the substrate by extraction of acidified reaction mixtures with ethyl acetate. Assay results for human serum angiotensin-converting enzyme can be obtained within 1.5 h of receipt of serum samples. Within the limits tested, the assay appears to be specific. However, interference by hitherto unrecognized enzymes of abnormal sera must be ruled out.

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