Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2010 Jun 5;401(2):190-6.
doi: 10.1016/j.virol.2010.01.033. Epub 2010 Mar 15.

Genetic characterization of HIV-1 from semen and blood from clade C-infected subjects from India and effect of therapy in these body compartments

Chengli Shen  1 , Ming Ding, Jodi K Craigo, Patrick Tarwater, Ramdas Chatterjee, Pratima Roy, Subhasish K Guha, Bibhuti Saha, Dolonchapa Modak, Dhrubak Neogi, Yue Chen, Phalguni Gupta
Affiliations

Genetic characterization of HIV-1 from semen and blood from clade C-infected subjects from India and effect of therapy in these body compartments

Chengli Shen et al. Virology. .

Abstract

Biologic and genetic differences between HIV-1 clade C in India and clade B in US suggest that the effect of anti-viral therapy in various body compartments may differ between these two clades. We examined the effect of therapy on viral loads in semen and blood of HIV-1-clade C infected subjects from India and evaluated whether HIV-1 in the semen is different from that in blood in these subjects. HIV-1 RNA was detected in semen and blood at all stages of the disease. Viral loads in semen and blood were strongly correlated with each other, but not with the CD4+ T cell count. Anti-viral treatment reduced viral load drastically in blood and semen within one month of post therapy. Genetic characterization of HIV-1 in the semen and blood demonstrated that they were highly compartmentalized. These data have important implications of sexual transmission of HIV-1 in clade C HIV-1 infected subjects.

PubMed Disclaimer

Figures

Figure 1
Figure 1. Viral load of bloods plasma and seminal plasma before and after potent antiretroviral therapy
Figure 2
Figure 2. Diversity and distance analysis of envelope sequences (C2–V5) of HIV-1 from Indian patients using PAUP* software
(A) Nucleic acid diversity of corrected intrapopulation diversity was calculated for nucleotide sequences. The quartile (box) differences between the diversity of all sequence clones were plotted as interpopulation distances as a function of different compartments. (B) Corrected population distance between blood derived plasma and cells and semen derived plasma and cells was calculated for nucleotide sequences. Statistical significance of the differences between different compartments for each analysis was performed using nonparametric statistics, the Mann-Whitney test. BP, blood plasma; BC, blood cells; SP, semen plasma; SC, semen cells. Blood compartment, distance between blood plasma and blood cells; Semen compartment, distance between seminal plasma and seminal cells.
Figure 3
Figure 3. Immune selection analysis of subgenome of C2-V5
Analysis of immune selection at various subgenomic regions (C2, V3, C3, V4, C4, and V5) of the whole C2-V5 sequences from four body compartments (seminal cells and plasma, and blood cells and plasma).
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Barroso PF, Schechter M, Gupta P, Bressan C, Bomfim A, Harrison LH. Adherence to antiretroviral therapy and persistence of HIV RNA in semen. J Acquir Immune Defic Syndr. 2003;32(4):435–440. - PubMed
    1. Barroso PF, Schechter M, Gupta P, Melo MF, Vieira M, Murta FC, Souza Y, Harrison LH. Effect of antiretroviral therapy on HIV shedding in semen. Ann Intern Med. 2000;133(4):280–284. - PubMed
    1. Byrn RA, Kiessling AA. Analysis of human immunodeficiency virus in semen: indications of a genetically distinct virus reservoir. Journal of Reproductive Immunology. 1998;41(1–2):161–176. - PubMed
    1. Cassol S, Weniger BG, Babu PG, Salminen MO, Zheng X, Htoon MT, Delaney A, O'Shaughnessy M, Ou CY. Detection of HIV type 1 env subtypes A, B, C, and E in Asia using dried blood spots: a new surveillance tool for molecular epidemiology. AIDS Research & Human Retroviruses. 1996;12(15):1435–1441. - PubMed
    1. Cecilia D, Kulkarni SS, Tripathy SP, Gangakhedkar RR, Paranjape RS, Gadkari DA. Absence of coreceptor switch with disease progression in human immunodeficiency virus infections in India. Virology. 2000;271(2):253–258. - PubMed

Publication types

MeSH terms

Associated data