doi: 10.1007/BF02223293.
Methods for the study of the glycoproteins and proteoglycans of bone using bacterial collagenase. Determination of bone sialoprotein and chondroitin sulphate
- PMID: 202375
- DOI: 10.1007/BF02223293
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Methods for the study of the glycoproteins and proteoglycans of bone using bacterial collagenase. Determination of bone sialoprotein and chondroitin sulphate
Calcif Tissue Res.
.
Abstract
A method has been developed for the solubilization of bone matrix using bacterial collagenase. The soluble macromolecules were separated by a column procedure on DEAE-cellulose and analysed for uronic and sialic acids. Values for the total non-collagenous fraction of bone and its content of bone sialoprotein and chondroitin sulphate were obtained. The method was compared with similar procedures using EDTA extraction and papain digestion.
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