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. 1977 Dec 14;24(1):29-36.
doi: 10.1007/BF02223293.

Methods for the study of the glycoproteins and proteoglycans of bone using bacterial collagenase. Determination of bone sialoprotein and chondroitin sulphate

G M Herring

Methods for the study of the glycoproteins and proteoglycans of bone using bacterial collagenase. Determination of bone sialoprotein and chondroitin sulphate

G M Herring. Calcif Tissue Res. .

Abstract

A method has been developed for the solubilization of bone matrix using bacterial collagenase. The soluble macromolecules were separated by a column procedure on DEAE-cellulose and analysed for uronic and sialic acids. Values for the total non-collagenous fraction of bone and its content of bone sialoprotein and chondroitin sulphate were obtained. The method was compared with similar procedures using EDTA extraction and papain digestion.

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References

    1. Arch Oral Biol. 1968 May;13(5):509-25 - PubMed
    1. Biochemistry. 1971 Mar 16;10(6):988-94 - PubMed
    1. Biochem J. 1973 Sep;136(1):125-34 - PubMed
    1. Biochim Biophys Acta. 1965 Nov 1;101(3):327-35 - PubMed
    1. Arch Oral Biol. 1971 Apr;16(4):411-21 - PubMed